Peer-Reviewed Scientific Publications
Total Citations >5,000 H-index- 18, I-10 index- 22 (UPDATED Feb, 2018)
Corresponding Author (where underlined)
- Hussain S*, Johnson CG*, Sciurba J*, Cyphert JM, Stober VP, Rice AB, Bulek K, Liu C, Aloor J, Gowdy K, Foster WM, Hollingsworth JW, Fessler MB, Li X, Tighe RM, Garantziotis S. TLR5 participates in TLR4 signaling and biases towards MyD88 activation in environmental lung injury Under revision Cell Reports
- Snyder R, Hussain S, Randell SH, Tucker CJ and Garantziotis S. Impaired ciliogenesis in differentiating human bronchial epithelia exposed to multi-walled carbon nanotubes. Part Fibre Toxicol. 2017, 13;14(1):44.
- Hilton MG, Taylor AJ, Hussain S, Dandley EC, Griffith EH, Garantziotis S, Parson GN, Bonner JC and Bereman MS. Mapping Differential Cellular Protein Response of Mouse Alveolar Epithelial Cells to Multi-Walled Carbon Nanotubes as a Function of Atomic Layer Deposition Coating. Nanotoxicology 2017 11(3):313-326
- Hussain S, Ji Z, Taylor AJ, Miller-DeGraff L, George M, Tucker J, Chang CH, Li R, Bonner JC, Garantziotis S. Multi- walled Carbon Nanotube Functionalization with High Molecular Weight Hyaluronan Significantly Reduces Pulmonary Injury. ACS Nano 2016. 10(8):7675-88. (Best Publication Award by the Nanotox Specialty section of the SOT)
- Hussain S, Kodavanti PP, Marshburn JD, Janoshazi A, Marinakos SM, George M, Rice A, Wiesner MR and Garantziotis S. Decreased Uptake and Enhanced Mitochondrial Protection Underlie Reduced Toxicity of Nanoceria in Human Macrophages Compared to Monocytes. 2016. J. Biomed. Nanotechnol. 2016. 12, 2139– 2150
- Kolinsky DJ,..,Hussain S,.., Zughaier SM, Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) Autophagy 2016;12(1):1-222.
- Guadagnini R, Moreau K, Hussain S, Marano F, Boland S. Interaction of nanoparticles used in medical applications with lung epithelial cells: cytotoxicity, oxidative stress and inflammatory response. Nanotoxicology 2015, 9 S1:25-32
- Hussain S, Sangtian S, Anderson SM, Snyder RJ, Marshburn JD, Rice AB, Bonner J, Garantziotis S. Inflammasome Activation in Airway Epithelial Cells after Multi-Walled Carbon Nanotube Exposure Mediates a Profibrotic Response in Lung Fibroblasts. Part Fibre Toxicol. 2014, 11:28.
- Hussain S, Garantziotis S, Rodrigues-Lima F, Dupret JM, Baeza-Squiban A, Boland S. Intracellular signal modulation by nanomaterials. Adv Exp Med Biol. 2014, 811:111-34.
- Snyder R, Hussain S, Rice A, Garantziotis S. Multi-Walled Carbon Nanotubes Induce Altered Morphology and Loss of Barrier Function in Human Bronchial Epithelia at Non-Cytotoxic Doses. Int. J. Nanomedicine 2014, 9:4093-105
- Boland S, Hussain S, Baeza-Squiban A, "Carbon Black and Titanium Dioxide Nanoparticles Induce Distinct Molecular Mechanisms of Toxicity" Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2014, 6(6):641-52
- Yokel RA, Hussain S, Garantziotis S, Demokritou P, Castranova V and Cassee F. The Yin: An adverse health perspective of nanoceria: uptake, distribution, accumulation, and the mechanisms of its toxicity. Environ. Sci.: Nano 2014, 1(5):406-428.
- Taylor AJ, McClure C, Shipley-Philips JK, Thompson EA, Hussain S, Garantziotis S, Parsons GN, Bonner JC. Atomic Layer Deposition Coating of Carbon Nanotubes with Aluminum Oxide Alters Pro-Fibrogenic Cytokine Expression by Human Mononuclear Phagocytes In vitro and Reduces Lung Fibrosis in Mice In vivo PLOS One 2014. 9(9):e106870
- Hussain S and Garantziotis S. Interplay between apoptotic and autophagy pathways after exposure to cerium dioxide nanoparticles in human monocytes. Autophagy. 2013. 9(1):101-103.
- Hussain S, Vanoirbeek J, Boland S, Haenen S, Marano F, Nemery B, Hoet PHM. Local lung inflammation leads to targeting of distinct organs by gold nanoparticles. BioMed Res Int 2013:923475
- Hussain S, Al-Nsour F, Rice A, Marshburn J, Ji Z, Zink I, Walker N, Garantziotis S. Cerium dioxide nanoparticles induce cell death in human peripheral blood monocytes through apoptosis and autophagy. ACS Nano. 2012. 6(7):5820-5829. (publication highlighted on NIEHS and NIH website)
- Hussain S, Vanoirbeek J, Boland S, Marano F, Nemery B, Hoet PHM. Nanoparticles modulate in vivo chemical sensitization. Part Fibre Toxicol. 2012. 9:15
- Hussain S, Al-Nsour F, Rice AB, Marshburn J, Ji Z, Zink JI, Yingling B, Walker NJ, Garantziotis S. Cerium dioxide nanoparticles do not modulate the lipopolysaccharide-induced inflammatory response of human monocytes. Int. J. Nanomedicine 2012. 2; 7:1387-97
- Hussain S, Vanoirbeek J, Hoet P. Interactions of nanomaterials with the immune system. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2012. 4(2):169-83.
- Hussain S, Jeroen JAJ, Luyts K, De Vooght V, Verbeken E, Boland S, Marano F, Nemery B, Hoet PHM. Lung exposure to nanoparticles modulates an asthmatic response in a mouse model of asthma. Eur Respir J. 2011. 37(2):299-309. (publication highlighted on Journal Cover)
- Sanfins E, Dairou J, Hussain S, Busi F, Chaffotte A, Rodrigues-Lima F and Dupret JM. Carbon black nanoparticles impair acetylation of aromatic amine carcinogens through inactivation of arylamine N-acetyltransferase enzymes ACS Nano. 2011. 5(6) :4504-4511
- Marano F, Hussain S, Rodrigues-Lima F, Baeza-Squiban A and Boland S. Nanoparticles: molecular targets and cell signaling. Arch Toxicol 2011. 85:733–741.
- Boland S, Guadagnini R, Baeza-Squiban A, Hussain S, Marano F. Nanoparticles used in medical applications for the lung: hopes for nanomedicine and fears for nanotoxicity. J. Phys.: Conf. Ser. 2011. 304: 012031
- Hussain S, Thomson CJL, Ferecatu I, Borot C, Andreau K, Martens JA, Fleury J, Baeza-Squiban A, Marano F and Boland S. Carbon Black and Titanium Dioxide Nanoparticles Elicit Distinct Apoptotic Pathways in Bronchial Epithelial Cells. Part Fibre Toxicol. 2010. 7:10
- Val S, Hussain S*, Boland S, Hamel R, Baeza-Squiban A, Marano F. Carbon black and titanium dioxide nanoparticles induce pro-inflammatory responses in bronchial epithelial cells: need for multiparametric evaluation due to adsorption artifacts. Inhal Toxicol. 2009. 21: Sl 1:115-22. *Equal contribution first authors
- Hussain S, Boland S, Baeza-Squiban A, Hamel R, Thomassen LCJ, Martens JA, Billon-Galland MA, Fleury-Feith J, Moisan F, Pairon JC, Marano F. Oxidative stress and proinflammatory effects of carbon black and titanium dioxide nanoparticles: Role of particle surface area and internalized amount. Toxicology 2009. 260, 142–149.
- Hussain S, Khan MZ, Khan A, Javed I, Asi MR. Toxico-pathological effects in rats induced by concurrent exposure to aflatoxin and cypermethrin. Toxicon 2009. 53, 33–41.
- Hussain S, I. Ali, A. Khan. Hematological and Plasma Proteins Findings in Fayoumi Hen Suffering from ascites.
Pak Vet. J. 2004. 24(4), 203-204.
Salik Hussain DVM, PhD
Dr. Hussain’s research group focuses on elucidating the molecular mechanisms of environmental disease susceptibility by delineating regulatory signaling networks involved in the susceptibility to environmental stressors including nanomaterials, particulate matter and ozone. We are interested in elaborating the pulmonary health effects of various environmental and occupational toxicants using translation relevant primary human cell models as well as rodent inhalation exposures. We have previously studied impacts of titanium dioxide, gold, cerium dioxide, carbon nanotubes and mycotoxins using disease animal models (occupational asthma and skin sensitization) and 3D primary human cell models from healthy and disease individuals.
Theme 1: Mechanistic and Translational NanoBiology
- Mechanisms of Programmed Cell Death Pathway Activation by Environmental Exposures
Programmed cell death pathways play a critical role in dictating the pathophysiology of environmental diseases. Over the past decade we have elaborated environmental exposures mediated programmed cell death pathways activation using various translational in vitro and in vivo models. For future studies, we are interested in delineating the cross-talk between various cell survival and injury pathways and mitochondrial dynamics after environmental exposures.
- 3-Dimensional Translational Models of Human Lung Epithelium
We work on 3-dimensional models of primary human and murine airways cultured at air-liquid interface to study the molecular mechanisms initiated by environmental exposures. We are interested in studying the modulation of airway differentiation and use it as a translational model to elaborate mechanisms of environmental injury in healthy and disease conditions.
- Application of Safety by Design Approach to Nanomaterial Risk Assessment
We are studying biocompatibility of novel nanomaterials for biomedical applications. We have previously employed functionalization of multi-walled carbon nanotubes (MWCNT) with high molecular weight hyaluronan (HA), a naturally occurring extracellular matrix sugar with excellent safety profile and significant anti-inflammatory properties, as a novel way to reduce pulmonary toxicity of MWCNT (Hussain et.al. ACS Nano 2016). We are now elaborating the role of different physico-chemical characteristics and other forms of surface functionalization’s and in delineating the role of mitochondrial injury and different intra-cellular signaling pathways.
Theme 2: Modulation of Disease Susceptibility by Environmental Exposures
We are studying the impacts of particulate exposures on the pathophysiology of pulmonary diseases and metabolic syndrome. We have previously demonstrated significant impacts of nanomaterial exposures on asthmatic responses as well as skin sensitization in rodents. We are currently exploring the mechanistic basis of these mechanisms using translational primary human cell models from health and disease individuals. We have shown the significant contribution of innate immune mechanisms particularly originating from Toll-Like Receptors in changing susceptibility to environmental disease like Asthma, fibrosis, COPD and metabolic syndrome.
Theme 3: Gestational and early life origins of adult onset disease
We are currently elaborating developmental origins of pulmonary diseases by studying the impacts of gestational and early life exposures to inhaled environmental stressors. We are employing individual as well as co-exposure scenarios and are studying the intricate interplay of cell survival and injury pathways as well as genetic and epigenetic mechanisms that govern susceptibility.
- Inhalation Exposures (particles, ozone etc)
- Disease Animal Models (Asthma, Fibrosis, COPD, Contact Sensitivity)
- Airway Physiology Measurements (Flexivent System)
- Primary Cell Cultures (Human and Rodent)
- 3-D Airway Epithelial Cell Models (Human and Rodent)
- Isolation, Culture and Study of Various Cell Types from Lungs, Blood and Bone Marrow
- Transgenic, floxed and conditionally KO mouse models
- Translational Studies
Grants and Research
We are currently seeking outstanding graduate and undergraduate students to join our lab! Interested individuals should contact Salik Hussain for details.
Post Doctoral Fellow - Lung Cell Biology and Physiology (08992)
Department of Physiology and Pharmacology, West Virginia University
Morgantown, West Virginia
The Department of Physiology and Pharmacology at West Virginia University School of Medicine is seeking applications for a Postdoctoral Fellow. This position is responsible for a translational project to elucidate the role of innate immune signaling in lung injury and repair. Current research is focused on the understanding of molecular and cellular basis of human pulmonary disease susceptibility after inhalation exposures to environmental stressors (xenobiotic particles and gases).
The successful candidate will be required to perform experimental work, assist with the writing of proposals to seek funding, develop new laboratory protocols and methods, perform independent experimental data analysis, oversee/direct, and assist undergraduate and graduate researchers in the completion of experiments. This postdoctoral fellow will also be responsible for maintaining documentation and detailing progress, to include detailed logs of work related activities and accurate records and data on research methods, procedures, and results. Reading scientific literature to keep abreast of technical advances and current research findings, attending research conferences, and writing and presenting research/study status reports for review and use of the Principle Investigator are also required as is maintaining, monitoring, and insuring proper use and conditions of lab equipment and keeping accurate inventory of lab assets.
A PhD in a relevant biomedical field (toxicology, cell and molecular biology, immunology, developmental biology, regenerative medicine) and hands on experience in animal models of pulmonary diseases are required. Additionally, the successful candidate will be skilled in using scientific rules and methods to solve problems; and identifying complex problems and reviewing related information to develop and evaluate options and implement solutions. The applicant should be able to collaborate effectively with a multidisciplinary team of co-investigators from diverse disciplines, orientations, and organizations. One year experience in each of the following is required as well:
- Rodent handling/surgeries and the ability to work with mice, including breeding and dosing
- Molecular biology skills and competency, such as Real-time PCR, and Western Blots
- Mammalian cell culture • Immune assays (such as ELISA)
- Flow cytometry/cell-sorting • Immunohistochemistry
Interested candidates should submit a cover letter, statement of research interests/goals, a current CV, and contact information for three (3) references via our online system at: https://wvu.taleo.net/careersection/staff/jobdetail.ftl?job=08992&tz=GMT-04%3A00hanks
For further information regarding the scientific nature of the position only, contact Salik Hussain, PhD at email@example.com
This position is subject to a background check. Salary will be based upon experience and NIH salary levels.
About West Virginia University:
WVU is the State’s comprehensive Land Grant University with an enrollment of about 29,000 students and a R1 Carnegie Classification. Morgantown and vicinity has a diverse population of about 62,000 residents and is ranked highly among the most livable small cities in the country. The community lies within a high technology corridor that also includes several federal research facilities as well as industries. The city is readily accessible and within driving distance from Pittsburgh, PA and Washington, DC.
West Virginia University is an Equal Opportunity/Affirmative Action Employer and the recipient of an NSF ADVANCE award for gender equity. The University values diversity among its faculty, staff and students, and invites applications from all qualified individuals, including minorities, females, individuals with disabilities, and veterans.