Hussain

Salik Hussain, DVM, PhD

Associate Professor

Contact

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Phone
304-293-8047
Fax
304-293-3850
Address
PO Box 9229
3074 RCB Health Sciences Center North
Morgantown, WV 26506-9229
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Positions

Associate Professor

Organization:
School of Medicine
Classification:
Faculty

Adjunct Associate Professor

Organization:
School of Medicine
Classification:
Faculty

Publications

Peer-Reviewed Scientific Publications

TOTAL PUBLICATIONS-50, Total Citations >18,000 H-index- 30, i10-index 49(February 2025) https://scholar.google.com/citations?user=wzO8aTMAAAAJ&hl=en

2025

  1. Sakamachi Y, Solis A, Johnson CG, Xiaoling M, Hussain S, O’Dwyer DN, Mehta S, Trempus CS, Thomas SY, Li JL, Karmaus P, McGrath J, Gibson K, Gleiberman A, Walts A, Invernizzi R, Molyneaux PL, Yang I, Zhang Y, Kaminski N, Schwartz DA, Gudkov A, Garantziotis S. Toll-Like-Receptor 5 protects against pulmonary fibrosis by reducing lung dysbiosis. bioRxiv [Preprint]. 2024 PMID: 39605370
     
  2. Chronic cigarette smoke exposure masks pathological features of Helicobacter pylori infection while promoting tumor initiation. Morris MT, Duncan BC, Piazuelo MB, Olfert IM, Xu X, Hussain S, Peek RM, Busada JT. Cancer Prev Res (Phila). 2025 PMID: 39789851

 

2024

  1. DeVallance E, Bowdridge E, Garner K, Griffith J, Seman M, Batchelor T, Velayutham M, Goldsmith T, Hussain S, Kelley EE, and Nurkiewicz TR. Alarmin IL-33 increases vascular tone via ERK-mediated Ca2+ sensitization and eNOS inhibition. J Physiol. 2024 Nov;602(22):6087-6107.
     
  2. Mazumder M.H.H, and Hussain S, Air pollution-mediated microbial dysbiosis in health and disease: Lung-gut axis and beyond. 2024. J Xenobiot. 14(4):1595-1612.
     
  3. Hussain S, Majumder N, Mazumder MH, Olapeju O, Amin MS, Brundage K, Velayutham M, Kelley EE, Vanoirbeek J. Ozone and house dust mite allergen co-exposure cause adverse asthmatic phenotype in a mouse model. Redox Biol. 2024. 76; 103330.
     
  4. Krystal A. Hughes, Bishal Misra, Maryam Maghareh, Parinya Samart, Ethan Nguyen, Salik Hussain, Werner J. Geldenhuys, Sharan Bobbala. Flash nanoprecipitation allows easy fabrication of pH-responsive acetalated dextran nanoparticles for intracellular release of payloads. Discov Nano. 2024 Jan 4;19(1):4.

 

2023

  1. Mazumder MHH, Gandhi J, Majumder N, Wang L, Cumming RI, Stradtman S, Velayutham M, Hathaway QA, Shannahan J, Hu G, Nurkiewicz TR, Tighe RM, Kelley EE, Hussain S. Lung-gut axis of microbiome alterations following co-exposure to ultrafine carbon black and ozone. Part Fibre Toxicol. 2023 Apr 21;20(1):15.
     
  2. Griffith JA, Dunn A, DeVallance E, Schafner KJ, Engles KJ, Batchelor TP, Goldsmith WT, Wix K, Hussain S, Bowdridge EC, Nurkiewicz TR. Maternal nano-titanium dioxide inhalation alters fetoplacental outcomes in a sexually dimorphic manner. Front Toxicol. 2023 Mar 6;5:1096173.
     
  3. Dunigan-Russell K, Yaeger MJ, Hodge M, Kilburg-Basnyat B, Reece SK, Birukova A, Guttenberg MA, Novak C, Chung S, Ehrmann BM, Diane Wallace E, Tokarz D, Majumder N, Li X, Christman JW, Shannahan J, Ballinger M, Hussain S, Shaikh SM, Tighe RM, Gowdy KM. Scavenger receptor BI attenuates oxidized phospholipid induced pulmonary inflammation Toxicol Appl Pharmacol. 2023 Jan 18:116381.
     
  4. Majumder N., Kodali V, Murugesan V, Goldsmith T, Amedro J, Erdely E, Kelley EE, Nurkiewicz TR, Hussain S.  Aerosol physico-chemical determinants of carbon black and ozone inhalation co-exposure induced pulmonary toxicity. Toxicol. Sci. 2023.  191 (1). 61-78.
     
  5. Majumder N, Deepak V, Hadique S, Aesoph D, Velayutham M, Ye Q, Mazumder MH, Lewis SE, Kodali V, Roohollahi A, Guo NL, Hu M, Khramtsov VV, Johnson RJ, Wen S, Kelley EE, Hussain S. Redox Imbalance in COVID-19 Pathophysiology 2022 Redox Biol. 56; 102465

 

2022

  1. Kodali V, Afshari A, Meighan T, McKinney W, Mazumder Md Habibul Hasan, Majumder Nairrita, Cumpston JL, Leonard HD, Cumpston JB, Friend S, Leonard SS, Erdely A, Zeidler-Erdely PC, Hussain S, Lee EG, Antonini JM. In vivo and in vitro toxicity of a stainless-steel aerosol generated during thermal spray coating. Arch Toxicol. 2022 Aug 19. doi: 10.1007/s00204-022-03362-7. PMID: 35984461.
     
  2. Trembley JH, So SW, Nixon JP, Bowdridge EC, Garner KL, Griffith J, Engles KJ, Batchelor TP, Goldsmith WT, Tomáška JM, Hussain S, Nurkiewicz TR, Butterick TA. Whole-body inhalation of nano-sized carbon black: a surrogate model of military burn pit exposure. BMC Res Notes. 2022 Aug 11;15(1):275.
     
  3. JA Griffith, KL Garner, E DeVallance, EC Bowdridge, WT Goldsmith, K Vix, S Hussain, TR Nurkiewicz. Impact of Nanoparticle Inhalation during Gestation on Cyclooxygenase Metabolites. 2022. Toxicol. Sci. 188(2):219-233.
     
  4. Bowdridge EC, DeVallance E, Garner KL, Griffith J, Schafner K, Seaman M, Engels K, Wix K, Batchelor TP, Goldsmith WT, Hussain S, and Nurkiewicz TR. Maternal engineered nanomaterial inhalation during gestation drives redox dysregulation and vascular dysfunction across generations. 2022. Part Fibre Toxicol. 19:18.

 

2021

  1. Hathaway QA, Majumder N, Kunovac A, Xie S, Pinti MV, Harkema JR, Nurkiewicz T, Hollander JM, Hussain S, Transcriptomics of Single Dose and Repeated Carbon Black and Ozone Inhalation Co-exposure Highlight Progressive Pulmonary Mitochondrial Dysfunction. 2021. Part Fibre Toxicol. 2021, 18:44.
     
  2. Majumder N, Velayutham M, Bitounis D, Kodali VK, Md. Habib-ul Hasan Mazumder, Amedro J, Khramtsov VA, Erdely A, Nurkiewicz TR, Demokritou P, Kelley EE, Hussain S. Oxidized carbon black nanoparticles induce endothelial damage through C-X-C chemokine receptor 3-mediated pathway, Redox Biol. 2021 4; 47:102161. Publication of the Year Award by NAMSS/SOT
     
  3. Majumder N, Goldsmith WT, Kodali VK, Velayutham M, Friend SA, Khramtsov VA, Nurkiewicz TR, Erdely A, Zeidler-Erdely PC, Castranova V, Harkema JR, Kelley EE, Hussain S. Oxidant-induced epithelial alarmin pathway mediates lung inflammation and functional decline following ultrafine carbon and ozone inhalation co-exposure, 2021. Redox Biol. 46:102092.
     
  4. Kolinsky DJ,..,Hussain S,.., Zughaier SM, Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) Autophagy. 2021 17(1):1-382.

 

2020

  1. Taylor-Just AJ, Ihrie MA, Duke KS, Lee HY, You DJ, Hussain S, Kodali VK, Ziemann C, Creutzenberg O, Vulponi A, Turcu F, Potara M, Todea M, Van den Brule S, Lison D, Bonner JC. The pulmonary toxicity of carboxylated or aminated multi-walled carbon nanotubes in mice is determined by the prior purification method. Part Fiber Toxicol. 17 (1):60 2020 PMID;33243293
     
  2. Hussain S, Johnson CG, Sciurba J, Cyphert JM, Stober VP, Rice AB, Bulek K, Liu C, Aloor J, Gowdy K, Foster WM, Hollingsworth JW, Fessler MB, Li X, Tighe RM, Garantziotis S. 2019 TLR5 participates in TLR4 signaling and biases towards MyD88 activation in environmental lung injury. Elife. 2020 Jan 28;9. pii: e50458.
     
  3. Whitehead GS, Hussain S, Fannin R, Innes CL, Schurman SH, Cook DN, Garantziotis S. 2019 TLR5 activation through flagellin promotes airway inflammation in asthma. Lung. 2020 Apr;198(2):289-298  

 

2019

  1. Mohammadinejad R, Moosavi MA, Tavakol S, Vardard DO, Hosseini V, Rahmati M, Dinig M, Hussain S, Mandegary A, Klionskyj D. Necrotic, apoptotic and autophagic cell fates triggered by nanoparticles Autophagy. 2019 15:4-33.

 

2017

  1. Snyder R, Hussain S, Randell SH, Tucker CJ and Garantziotis S. Impaired ciliogenesis in differentiating human bronchial epithelia exposed to multi-walled carbon nanotubes. Part Fibre Toxicol. 2017, 13; 14(1):44.
     
  2. Hilton MG, Taylor AJ, Hussain S, Dandley EC, Griffith EH, Garantziotis S, Parson GN, Bonner JC and Bereman MS. Mapping Differential Cellular Protein Response of Mouse Alveolar Epithelial Cells to Multi-Walled Carbon Nanotubes as a Function of Atomic Layer Deposition Coating. Nanotoxicology 2017 11(3):313-326

 

2016

  1. Hussain S, Ji Z, Taylor AJ, Miller-DeGraff L, George M, Tucker J, Chang CH, Li R, Bonner JC, Garantziotis S. Multi- walled Carbon Nanotube Functionalization with High Molecular Weight Hyaluronan Significantly Reduces Pulmonary Injury. ACS Nano 2016. 10(8):7675-88.
     
  2. Hussain S, Kodavanti PP, Marshburn JD, Janoshazi A, Marinakos SM, George M, Rice A, Wiesner MR and Garantziotis S. Decreased Uptake and Enhanced Mitochondrial Protection Underlie Reduced Toxicity of Nanoceria in Human Macrophages Compared to Monocytes. 2016. J. Biomed. Nanotechnol. 2016. 12, 2139– 2150
     
  3. Kolinsky DJ,..,Hussain S,.., Zughaier SM, Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) Autophagy 2016;12(1):1-222.
     

2015

  1. Guadagnini R, Moreau K, Hussain S, Marano F, Boland S.  Interaction of nanoparticles used in medical applications with lung epithelial cells: cytotoxicity, oxidative stress and inflammatory response. Nanotoxicology 2015, 9 S1:25-32

 

2014

  1. Hussain S, Sangtian S, Anderson SM, Snyder RJ, Marshburn JD, Rice AB, Bonner J, Garantziotis S. Inflammasome Activation in Airway Epithelial Cells after Multi-Walled Carbon Nanotube Exposure Mediates a Profibrotic Response in Lung Fibroblasts. Part Fibre Toxicol. 2014, 11:28.
     
  2. Hussain S, Garantziotis S, Rodrigues-Lima F, Dupret JM, Baeza-Squiban A, Boland S. Intracellular signal modulation by nanomaterials. Adv Exp Med Biol. 2014, 811:111-34.
     
  3. Snyder R, Hussain S, Rice A, Garantziotis S. Multi-Walled Carbon Nanotubes Induce Altered Morphology and Loss of Barrier Function in Human Bronchial Epithelia at Non-Cytotoxic Doses. Int. J. Nanomedicine 2014, 9:4093-105.
     
  4. Boland S, Hussain S, Baeza-Squiban A, "Carbon Black and Titanium Dioxide Nanoparticles Induce Distinct Molecular Mechanisms of Toxicity" Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2014, 6(6):641-52.
     
  5. Yokel RA, Hussain S, Garantziotis S, Demokritou P, Castranova V and Cassee F. The Yin: An adverse health perspective of nanoceria: uptake, distribution, accumulation, and the mechanisms of its toxicity. Environ. Sci.: Nano 2014, 1(5):406-428.
     
  6. Taylor AJ, McClure C, Shipley-Philips JK, Thompson EA, Hussain S, Garantziotis S, Parsons GN, Bonner JC. Atomic Layer Deposition Coating of Carbon Nanotubes with Aluminum Oxide Alters Pro-Fibrogenic Cytokine Expression by Human Mononuclear Phagocytes In vitro and Reduces Lung Fibrosis in Mice In vivo PLOS One 2014. 9(9):e106870.

 

2013

  1. Hussain S and Garantziotis S. Interplay between apoptotic and autophagy pathways after exposure to cerium dioxide nanoparticles in human monocytes. Autophagy. 2013. 9(1):101-103.
     
  2. Hussain S, Vanoirbeek J, Boland S, Haenen S, Marano F, Nemery B, Hoet PHM. Local lung inflammation leads to targeting of distinct organs by gold nanoparticles. BioMed Res Int (formerly Journal of Biomedicine and Biotechnology) 2013:923475.

 

2012

  1. Hussain S, Al-Nsour F, Rice A, Marshburn J, Ji Z, Zink I, Walker N, Garantziotis S. Cerium dioxide nanoparticles induce cell death in human peripheral blood monocytes through apoptosis and autophagy. ACS Nano. 2012. 6(7):5820-5829.
     
  2. Hussain S, Vanoirbeek J, Boland S, Marano F, Nemery B, Hoet PHM. Nano-titanium dioxide modulates the dermal sensitization potency of DNCB. Part Fibre Toxicol. 2012. 9:15.
     
  3. Hussain S, Al-Nsour F, Rice AB, Marshburn J, Ji Z, Zink JI, Yingling B, Walker NJ, Garantziotis S. Cerium dioxide nanoparticles do not modulate the lipopolysaccharide-induced inflammatory response of human monocytes. Int. J. Nanomedicine 2012. 2; 7:1387-97.
     
  4. Hussain S, Vanoirbeek J, Hoet P. Interactions of nanomaterials with the immune system. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2012. 4(2):169-83.

 

2011

  1. Hussain S, Jeroen JAJ, Luyts K, De Vooght V, Verbeken E, Boland S, Marano F, Nemery B, Hoet PHM. Lung exposure to nanoparticles modulates an asthmatic response in a mouse model of asthma. Eur Respir J. 2011. 37(2):299-309.
     
  2. Sanfins E, Dairou J, Hussain S, Busi F, Chaffotte A, Rodrigues-Lima F and Dupret JM. Carbon black nanoparticles impair acetylation of aromatic amine carcinogens through inactivation of arylamine N-acetyltransferase enzymes ACS Nano. 2011. 5(6) :4504-4511.
     
  3. Marano F, Hussain S, Rodrigues-Lima F, Baeza-Squiban A and Boland S. Nanoparticles: molecular targets and cell signaling. Arch Toxicol 2011. 85:733–741.
     
  4. Boland S, Guadagnini R, Baeza-Squiban A, Hussain S, Marano F. Nanoparticles used in medical applications for the lung: hopes for nanomedicine and fears for nanotoxicity. J. Phys.: Conf. Ser. 2011. 304: 012031.

 

2010

  1. Hussain S, Thomson CJL, Ferecatu I, Borot C, Andreau K, Martens JA, Fleury J, Baeza-Squiban A, Marano F and Boland S. Carbon Black and Titanium Dioxide Nanoparticles Elicit Distinct Apoptotic Pathways in Bronchial Epithelial Cells. Part Fibre Toxicol. 2010. 7:10

 

2009

  1. Val S, Hussain S*, Boland S, Hamel R, Baeza-Squiban A, Marano F.  Carbon black and titanium dioxide nanoparticles induce pro-inflammatory responses in bronchial epithelial cells: need for multiparametric evaluation due to adsorption artifacts. Inhal Toxicol. 2009. 21: Sl 1:115-22. *Equal contribution first authors
     
  2. Hussain S, Boland S, Baeza-Squiban A, Hamel R, Thomassen LCJ, Martens JA, Billon-Galland MA, Fleury-Feith J, Moisan F, Pairon JC, Marano F. Oxidative stress and proinflammatory effects of carbon black and titanium dioxide nanoparticles: Role of particle surface area and internalized amount. Toxicology 2009. 260, 142–149.
     
  3. Hussain S, Khan MZ, Khan A, Javed I, Asi MR. Toxico-pathological effects in rats induced by concurrent exposure to aflatoxin and cypermethrin. Toxicon 2009. 53, 33–41.

 

2004

  1. Hussain S, I. Ali, A. Khan. Hematological and Plasma Proteins Findings in Fayoumi Hen Suffering from ascites. Vet. J. 2004. 24(4), 203-204.

Research Interests

The Hussain Lab is a “Translational Nanotoxicology and Environmental Health Laboratory.” We study pulmonary and systemic health impacts of inhalation exposures/co-exposures to environmentally relevant particulates (Particulate Matter, Engineered Nanomaterials) and gases (O3, NOx etc.,). Areas of interest currently under investigation are

1) Inflammation, injury and repair of lungs in response to environmental insults

2) Lung and gut microbial dysbiosis after environmental exposures

3) Gene x Environment interaction in environmental lung injury

4) Systemic outcomes after gestational inhalation exposures

We utilize state of the art Pulmonary Function Measurements, Air-Liquid Interface Cultures, 3-D Organoids and disease animal models (genetic and acquired susceptibility) to elaborate organismal/cellular responses and mechanistic pathways after environmental exposures.  

 

More details about Dr. Hussain’s research program can be found at https://hussainlab.org/

 

​Techniques:

Inhalation Exposures (particles, ozone etc)

Disease Animal Models (Asthma, Fibrosis, COPD, Contact Sensitivity)

Airway Physiology Measurements (Flexivent System)

Primary Cell Cultures (Human and Rodent)

3-D Airway Epithelial Cell Models (Human and Rodent)

Isolation, Culture and Study of Various Cell Types from Lungs, Blood and Bone Marrow

Transgenic, floxed and conditionally KO mouse models

Translational Studies

Grants and Research

Current Funding:

2020-2026         

Impact of Particle and Ozone Inhalation Co-Exposure on Alveolar Epithelial Regeneration

NIH/NIEHS Outstanding New Environmental Scientist (ONES), Career Development Award/R01

Role: Principal Investigator

 

2023-2026        

Inflammatory cross-talk between heat events and air pollution

NIH/NIEHS CCI Supplement

Role: Principal Investigator 

 

2024-2028         

Combustion Source Mixtures: Characteristics and Investigation of Health Effects

NIOSH/NORA HELD009

Role: Collaborator

 

2023-2028        

Influence of particulate matter on fetal mitochondrial programming

NIH/NIEHS R01

Role: Co-Investigator (5 % Effort)

 

2022-2026        

Pre-doctoral Training in Systems Toxicology (SysTox)

NIH/NIEHS T32  (ES032920)

Role: Pulmonary Function Core Director and Training Preceptor

 

Completed Projects:

2022-2024

WVU Health Sciences Centre Synergy Grant- Systems toxicity of Inhalation exposures to single and complex mixtures

Role:  MPI

 

2020-2021

An association of NOD-like receptor (NLR) expression and single nucleotide polymorphism with susceptibility and severity of COVID-19   

U54 GM104942   (Hodder)  

Role: Principal Investigator - Pilot Project
 

2017-2022  

Faculty Startup Funding, West Virginia University School of Medicine 

PI: Salik Hussain, PhD

Additional Info

Postdoctoral Fellow - Physiology and Pharmacology (14910)

The Department of Physiology and Pharmacology in the West Virginia University School of Medicine is seeking applications for a Postdoctoral Fellow. This position is responsible for a translational project to elucidate the role of environmental exposures and innate immune signaling in lung injury and repair. Current research is focused on the understanding of molecular and cellular basis of pulmonary disease susceptibility after inhalation exposures to environmental stressors (xenobiotic particles and gases).

PhD in toxicology, cell and molecular biology, immunology, developmental biology, regenerative medicine, or a relevant biomedical field is required. One to two years of hands on experience in animal models of pulmonary diseases and/or cell culture techniques is required. This experience is typically acquired through the course of doctoral training.

Please submit 1) cover letter 2) current CV 3) three references (contact information).  To apply online https://wvu.taleo.net/careersection/staff/jobdetail.ftl?job=14910&tz=GMT-04%3A00&tzname=America%2FNew_York

For further information about the scientific details and responsibilities duties or responsibilities please contact Salik Hussain, PhD (salik.hussain@hsc.wvu.edu) and visit https://hussainlab.org/.

West Virginia University is an Equal Opportunity Employer and is the recipient of an NSF ADVANCE award for gender equity. The University values diversity among its faculty, staff, and students, and invites applications from all qualified applicants regardless of race, ethnicity, color, religion, gender identity, sexual orientation, age, nationality, genetics, disability, or veteran status.