- PhD, Peking University, 2006
Grenell A, Wang Y, Yam M, Swarup A, Dilan TL, Hauer A, Linton JD, Philp NJ, Gregor E, Zhu S, Shi Q, Murphy J, Guan T, Lohner D, Kolandaivelu S, Ramamurthy V, Goldberg AFX, Hurley JB, Du J. "Loss of MPC1 reprograms retinal metabolism to impair visual function". Proc Natl Acad Sci U S A. 2019 Feb 26;116(9):3530-3535. PMID: 3080874
Swarup A, Samuels IS, Bell BA, Han JYS, Du J, Massenzio E, Abel ED, Boesze-Battaglia K, Peachey NS, Philp NJ. "Modulating GLUT1 expression in the RPE decreases glucose levels in the retina: Impact on photoreceptors and Müller glial cells". Am J Physiol Cell Physiol. 2019; 316(1):C121-C133. PMID: 30462537.
Sinha T, Makia M, Du J, Naash MI, Al-Ubaidi MR. "Flavin homeostasis in the mouse retina during aging and degeneration." J Nutr Biochem. 2018 Sep 15;62:123-133. doi: 10.1016/j.jnutbio.2018.09.003. [Epub ahead of print] PMID: 30290331
Wang Y, Grenell A, Zhong F, Yam M, Hauer A, Gregor E, Zhu S, Lohner D, Zhu J, Du J. "Metabolic signature of the aging eye in mice." Neurobiol Aging. 2018 Aug 7;71:223-233. doi: 10.1016/j.neurobiolaging.2018.07.024. [Epub ahead of print] PMID: 30172221
Zhu S, Yam M, Wang Y, Linton JD, Grenell A, Hurley JB, Du J. "Impact of euthanasia, dissection and postmortem delay on metabolic profile in mouse retina and RPE/choroid." Exp Eye Res. 2018 Jun 1. pii: S0014-4835(18)30058-7. doi: 10.1016/j.exer.2018.05.032. PMID: 29864440.
Du J, An J, Linton JD, Wang Y, Hurley JB. "How Excessive cGMP Impacts Metabolic Proteins in Retinas at the Onset of Degeneration." Adv Exp Med Biol. 2018;1074:289-295. doi: 10.1007/978-3-319-75402-4_35. PMID: 29721955.
Swarup A, Bell BA, Du J, Han JYS, Soto J, Abel ED, Bravo-Nuevo A, FitzGerald PG, Peachey NS, Philp NJ. "Deletion of GLUT1 in mouse lens epithelium leads to cataract formation." Exp Eye Res. 2018 Mar 28. pii: S0014-4835(17)30668-1. doi: 10.1016/j.exer.2018.03.021. [Epub ahead of print] PMID:29604281
Zhang T, Gillies MC, Madigan MC, Shen W, Du J, Grünert U, Zhou F, Yam M, Zhu L. "Disruption of De Novo Serine Synthesis in Müller Cells Induced Mitochondrial Dysfunction and Aggravated Oxidative Damage." Mol Neurobiol. 2018 Jan 30. doi: 10.1007/s12035-017-0840-8. [Epub ahead of print]
Kanow MA, Giarmarco MM, Jankowski CS, Tsantilas K, Engel AL, Du J, Linton JD, Farnsworth CC, Sloat SR, Rountree A, Sweet IR, Lindsay KJ, Parker ED, Brockerhoff SE, Sadilek M, Chao JR, Hurley JB. "Biochemical adaptations of the retina and retinal pigment epithelium support a metabolic ecosystem in the vertebrate eye." Elife. 2017 Sep 13;6. pii: e28899. doi: 10.7554/eLife.28899.
Chao JR, Knight K, Engel AL, Jankowski C, Wang Y, Manson MA, Gu H, Djukovic D, Raftery D, Hurley JB, Du J. "Human Retinal Pigment Epithelium Cells prefer proline as a nutrient and transport metabolic intermediates to the retinal side." J Biol Chem. 2017 Aug 4;292(31):12895-12905
Metabolic signaling in healthy and diseased retina, metabolomics, patient-derived stem cells.
Description of Research
Metabolic network in the visual system
The eye consumes extremely high energy every second and the disturbance of its metabolism can cause blindness. Understanding its unique metabolism is fundamental for retinal degenerative diseases like inherited degeneration, age related macular degeneration (AMD) and diabetic retinopathy.
We are addressing several questions:
- How are nutrients transported and utilized in retinal cells?
- How do photoreceptor cells,glial cells and retinal pigment epithelium coordinate together to make metabolic circuits to maintain their specialized functions?
- How important are glycolysis, mitochondrial oxidative phosphorylation and reductive carboxylation in the retina?
We are using targeted metabolomics. metabolic flux analysis and cell-specific conditional knockout mice animals to test our models.
Targeting metabolism to fight blindness
Our goal is to reveal the metabolic basis for retinal degenerative diseases and to develop new approaches to slow down or prevent blindness. We are using human primary cultured cells, patient-derived retinal cells by iPS technology, genetically modified mouse.models and collaborating with clinicians to test our hypotheses with both targeted and untargeted metabolomics, biomechanical assays and live imaging. We will develop both CRISPR and small molecule-based approaches to attack the metabolic reprogramming in retinal disease