Mark Tseytlin, PhD

Associate Professor

Organization:

West Virginia University School of Medicine

Department:

Biochemistry and Molecular Medicine

Classification:

Faculty

Member

Organization:

West Virginia University WVU Cancer Institute

Department:

WVU Cancer Institute Research Programs

Classification:

Faculty

Oba M, Velayutham M, Tseytlin O, Eubank T, Driesschaert B, Khramtsov V, Hirata H, Tseytlin M Dynamic Monitoring of the Partial Pressure of Oxygen and pH in a Human Monocytic Cell Culture System Using Rapid-Scan Electron Paramagnetic Resonance Imaging with a Monophosphonated Trityl Spin Probe. Chemical & Biomedical Imaging 2026. 10.1021/cbmi.6c00111.

Tseytlin O, Sestito M, Eubank TD, Khramtsov VV, Driesschaert B, Boone BA, Tseytlin M. Reconfigurable animal bed-EPR resonator assembly for multimodal co-registration. J Magn Reson. 2026 Jan;382:107995. doi: 10.1016/j.jmr.2025.107995. Epub 2025 Nov 26. PMID: 41319401; PMCID: PMC12806151.

Tseytlin M, Tseytlin O. Computationally efficient 4D spectral-spatial EPR imaging. J Magn Reson. 2025 Dec;381:107980. doi: 10.1016/j.jmr.2025.107980. PMID: 41106136; PMCID: PMC12614930.

Sarvari S, McGee D, O'Connell R, Tseytlin O, Bobko AA, Tseytlin M. Electron Spin Resonance Probe Incorporation into Bioinks Permits Longitudinal Oxygen Imaging of Bioprinted Constructs. Mol Imaging Biol. 2024 Jun;26(3):511-524. doi: 10.1007/s11307-023-01871-0. PMID: 38038860; PMCID: PMC11211156.

Mihalik NE, Steinberger KJ, Stevens AM, Bobko AA, Hoblitzell EH, Tseytlin O, Akhter H, Dziadowicz SA, Wang L, O'Connell RC, Monaghan KL, Hu G, Mo X, Khramtsov VV, Tseytlin M, Driesschaert B, Wan ECK, Eubank TD.Dose-Specific Intratumoral GM-CSF Modulates Breast Tumor Oxygenation and Antitumor Immunity. J Immunol. 2023 Nov 15;211(10):1589-1604. doi: 10.4049/jimmunol.2300326. PMID: 37756529; PMCID: PMC10656117.

O'Connell RC, Tseytlin O, Bobko AA, Eubank TD, Tseytlin M. Rapid scan EPR: Automated digital resonator control for low-latency data acquisition. J Magn Reson. 2022 Dec;345:107308. doi: 10.1016/j.jmr.2022.107308. Epub 2022 Oct 21. PMID: 36356489; PMCID: PMC10266206.

Tseytlin O, O'Connell R, Sivashankar V, Bobko AA, Tseytlin M. Rapid Scan EPR Oxygen Imaging in Photoactivated Resin Used for Stereolithographic 3D Printing. 3D Print Addit Manuf. 2021 Dec 1;8(6):358-365. doi: 10.1089/3dp.2020.0170. Epub 2021 Dec 9. PMID: 34977276; PMCID: PMC8713732.

Tseytlin O, Bobko AA, Tseytlin M. Rapid Scan EPR imaging as a Tool for Magnetic Field Mapping. Appl Magn Reson. 2020 Oct;51(9-10):1117-1124. doi: 10.1007/s00723-020-01238-6. Epub 2020 Sep 25. PMID: 33642700; PMCID: PMC7909464.

Tseytlin M. General solution for rapid scan EPR deconvolution problem. J Magn Reson. 2020 Sep;318:106801. doi: 10.1016/j.jmr.2020.106801. Epub 2020 Aug 1. PMID: 32862080; PMCID: PMC7575242.

Sanzhaeva U, Poncelet M, Tseytlin O, Tseytlin M, Gencheva M, Eubank TD, Khramtsov VV, Driesschaert B. Synthesis, Characterization, and Application of a Highly Hydrophilic Triarylmethyl Radical for Biomedical EPR. J Org Chem. 2020 Aug 21;85(16):10388-10398. doi: 10.1021/acs.joc.0c00557. Epub 2020 Aug 6. PMID: 32698583; PMCID: PMC7814971.

Tseytlin O, Guggilapu P, Bobko AA, AlAhmad H, Xu X, Epel B, O'Connell R, Hoblitzell EH, Eubank TD, Khramtsov VV, Driesschaert B, Kazkaz E, Tseytlin M. Modular imaging system: Rapid scan EPR at 800 MHz. J Magn Reson. 2019 Aug;305:94-103. doi: 10.1016/j.jmr.2019.06.003. Epub 2019 Jun 8. PMID: 31238278; PMCID: PMC6656609.

Poncelet M, Driesschaert B, Tseytlin O, Tseytlin M, Eubank TD, Khramtsov VV. Dextran-conjugated tetrathiatriarylmethyl radicals as biocompatible spin probes for EPR spectroscopy and imaging. Bioorg Med Chem Lett. 2019 Jul 15;29(14):1756-1760. doi: 10.1016/j.bmcl.2019.05.017. Epub 2019 May 13. PMID: 31129052; PMCID: PMC6557268.

Sato-Akaba H, Tseytlin M. Development of an L-band rapid scan EPR digital console. J Magn Reson. 2019 Jul;304:42-52. doi: 10.1016/j.jmr.2019.05.003. Epub 2019 May 10. PMID: 31100585; PMCID: PMC7549020.

Sanzhaeva U, Xu X, Guggilapu P, Tseytlin M, Khramtsov VV, Driesschaert B. Imaging of Enzyme Activity by Electron Paramagnetic Resonance: Concept and Experiment Using a Paramagnetic Substrate of Alkaline Phosphatase. Angew Chem Int Ed Engl. 2018 Sep 3;57(36):11701-11705. doi: 10.1002/anie.201806851. Epub 2018 Aug 7. PMID: 30003653; PMCID: PMC6327950.

Tseytlin M, Stolin AV, Guggilapu P, Bobko AA, Khramtsov VV, Tseytlin O, Raylman RR. A combined positron emission tomography (PET)-electron paramagnetic resonance imaging (EPRI) system: initial evaluation of a prototype scanner. Phys Med Biol. 2018 May 16;63(10):105010. doi: 10.1088/1361-6560/aabfa1. PMID: 29676283; PMCID: PMC5979266.

Tseytlin M. Concept of Phase Cycling in Pulsed Magnetic Resonance Using Sinusoidal Magnetic Field Modulation. Z Phys Chem (N F). 2017 Mar;231(3):689-703. doi: 10.1515/zpch-2016-0843. Epub 2016 Nov 9. PMID: 28751814; PMCID: PMC5523863.

Tseytlin M. Full cycle rapid scan EPR deconvolution algorithm. J Magn Reson. 2017 Aug;281:272-278. doi: 10.1016/j.jmr.2017.06.008. Epub 2017 Jun 11. PMID: 28666168; PMCID: PMC5568913.

Möser J, Lips K, Tseytlin M, Eaton GR, Eaton SS, Schnegg A. Using rapid-scan EPR to improve the detection limit of quantitative EPR by more than one order of magnitude. J Magn Reson. 2017 Aug;281:17-25. doi: 10.1016/j.jmr.2017.04.003. Epub 2017 Apr 17. PMID: 28500917; PMCID: PMC5556260.

Khramtsov VV, Bobko AA, Tseytlin M, Driesschaert B. Exchange Phenomena in the Electron Paramagnetic Resonance Spectra of the Nitroxyl and Trityl Radicals: Multifunctional Spectroscopy and Imaging of Local Chemical Microenvironment. Anal Chem. 2017 May 2;89(9):4758-4771. doi: 10.1021/acs.analchem.6b03796. Epub 2017 Apr 10. PMID: 28363027; PMCID: PMC5513151.

Epel B, Sundramoorthy SV, Krzykawska-Serda M, Maggio MC, Tseytlin M, Eaton GR, Eaton SS, Rosen GM, Kao JPY, Halpern HJ. Imaging thiol redox status in murine tumors in vivo with rapid-scan electron paramagnetic resonance. J Magn Reson. 2017 Mar;276:31-36. doi: 10.1016/j.jmr.2016.12.015. Epub 2016 Dec 31. PMID: 28092786; PMCID: PMC5336491.

Biller JR, Mitchell DG, Tseytlin M, Elajaili H, Rinard GA, Quine RW, Eaton SS, Eaton GR. Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo. J Vis Exp. 2016 Sep 26;(115):54068. doi: 10.3791/54068. PMID: 27768025; PMCID: PMC5092072.

Tseytlin M, Epel B, Sundramoorthy S, Tipikin D, Halpern HJ. Decoupling of excitation and receive coils in pulsed magnetic resonance using sinusoidal magnetic field modulation. J Magn Reson. 2016 Nov;272:91-99. doi: 10.1016/j.jmr.2016.09.004. Epub 2016 Sep 7. PMID: 27673275; PMCID: PMC5071169.

Biomedical Imaging and Bioengineering

Research Interests

Development of novel bio-imaging methodologies, including theory, hardware and software.

The focus of our lab has been mostly on the development of novel electron paramagnetic resonance (EPR) imaging techniques. An alternatively used name is electron spin resonance (ESR). EPR is in many aspects similar to a better known nuclear magnetic resonance (NMR) modality. The same quantum mechanics laws govern both nuclear and electron spins. EPR is applied to solve medical and biological problems to a much less extent compared to NMR, and especially to its imaging modality magnetic resonance imaging (MRI). The major reason has been that protons (possess nuclear spin, S=1/2) in water are abundant in living organisms. Biomedical EPR requires the development of bio-compatible molecular probes that have to be injected or implanted into the biological object under investigation. Such probes have been developed worldwide. Our WVU collaborators Drs. Khramtsov, Bobko and Driesschaert are pioneers in the field of EPR probes synthesis. The major goal of our lab is to develop instrumentation and data processing methods that enable reliable extraction of functional information, e.g. oxygen concentration, within a limited experimental time. Compared to measurement of inanimate objects, animals and people cannot be held for a long time immobilized in the magnet. Imaging experiment lasts from 3 to 20 minutes. During this time, thousands of EPR spectra have to be measured to post-processed to form an image.

Students help winding wire around a coil that will be used in the EPR spectrometer.

A student is tuning EPR system before measurement.