Epel B, Sundramoorthy SV, Krzykawska-Serda M, Maggio MC, Tseytlin M, Eaton GR, Eaton SS, Rosen GM, J PYK, Halpern HJ. "Imaging thiol redox status in murine tumors in vivo with rapid-scan electron paramagnetic resonance." J Magn Reson. 2017;276:31-6. doi: 10.1016/j.jmr.2016.12.015. PubMed PMID: 28092786; PMCID: PMC5336491.
Tseytlin M. "Concept of Phase Cycling in Pulsed Magnetic Resonance Using Sinusoidal Magnetic Field Modulation." Z Phys Chem. 2017;231(3):689-703. doi: 10.1515/zpch-2016-0843. PubMed PMID: WOS:000393583200019, NIHMSID: NIHMS863707.
Tseytlin M. "Full Cycle Rapid Scan EPR Deconvolution Algorithm." J Magn Res. 2017; 281 (272)
Khramtsov VV, Bobko AA, Tseytlin M, Driesschaert B. "Exchange Phenomena in the Electron Paramagnetic Resonance Spectra of the Nitroxyl and Trityl Radicals: Multifunctional Spectroscopy and Imaging of Local Chemical Microenvironment." Anal Chem. 2017;89(9):4758-71. doi: 10.1021/acs.analchem.6b03796. PubMed PMID: 28363027.
Moser J, Lips K, Tseytlin M, Eaton GR, Eaton SS, Schnegg A. "Using rapid-scan EPR to improve the detection limit of quantitative EPR by more than one order of magnitude." J Magn Reson. 2017;281:17-25. doi: 10.1016/j.jmr.2017.04.003. PubMed PMID: 28500917.
Tseytlin M, Epel B, Sundramoorthy S, Tipikin D, Halpern HJ. "Decoupling of excitation and receive coils in pulsed magnetic resonance using sinusoidal magnetic field modulation." J Magn Reson. 2016;272:91-9. doi: 10.1016/j.jmr.2016.09.004. PubMed PMID: 27673275; PMCID: PMC5071169.
Biller JR, Mitchell DG, Tseytlin M, Elajaili H, Rinard GA, Quine RW, Eaton SS, Eaton GR. "Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo." J Vis Exp. 2016(115). doi: 10.3791/54068. PubMed PMID: 27768025; PMCID: PMC5092072.
Z. Yu, M. Tseytlin, S.S. Eaton, and G. R. Eaton, "Multiharmonic Electron Paramagnetic Resonance for Extended Samples with both Narrow and Broad Lines", J. Magn. Reson., 254, pp 86-92
Elajaili HB, Biller JR, Tseitlin M, Dhimitruka I, Khramtsov VV, Eaton SS, Eaton GR. "Electron spin relaxation times and rapid scan EPR imaging of pH-sensitive amino-substituted trityl radicals". Magn Reson Chem. 2015 Apr;53(4):280-4. doi: 10.1002/mrc.4193. Epub 2014 Dec 12. PMID: 25504559
Biller JR, Tseitlin M, Mitchell DG, Yu Z, Buchanan LA, Elajaili H, Rosen GM, Kao JP, Eaton SS, Eaton GR. "Improved sensitivity for imaging spin trapped hydroxyl radical at 250 MHz." Chemphyschem. 2015 Feb 23;16(3):528-31. doi: 10.1002/cphc.201402835. Epub 2014 Dec 8. PMID: 25488257
Tseitlin M, Yu Z, Quine RW, Rinard GA, Eaton SS, Eaton GR. "Digitally generated excitation and near-baseband quadrature detection of rapid scan EPR signals". J Magn Reson. 2014 Oct 30;249C:126-134. doi: 10.1016/j.jmr.2014.10.011. [Epub ahead of print] PMID: 25462956
Yu Z, Quine RW, Rinard GA, Tseitlin M, Elajaili H, Kathirvelu V, Clouston LJ, Boratyński PJ, Rajca A, Stein R, Mchaourab H, Eaton SS, Eaton GR. "Rapid-scan EPR of immobilized nitroxides". J Magn Reson. 2014 Oct;247:67-71. doi: 10.1016/j.jmr.2014.08.008. Epub 2014 Aug 30. PMID: 25240151
Tseitlin M, Biller JR, Elajaili H, Khramtsov VV, Dhimitruka I, Eaton GR, Eaton SS. "New spectral-spatial imaging algorithm for full EPR spectra of multiline nitroxides and pH sensitive trityl radicals". J Magn Reson. 2014 Aug;245:150-5. doi: 10.1016/j.jmr.2014.05.013. Epub 2014 Jun 17. PMID: 25058914 Free PMC Article
Nuclear Magnetic Resonance
My collaborators and I are developing new spectroscopic and imaging methods for in vivo Electron Paramagnetic Resonance (EPR). EPR is in many ways analogous to better known Nuclear Magnetic Resonance (NMR) and its imaging modality MRI. While NMR and MRI methods detect signals produced by nuclear spins in protons and other chemical elements, EPR measures electron spins in all kinds of free radicals. A variety of stable radicals has been synthesized that reports the physiological microenvironment in normal tissues and tumors. This includes measurements of pO2, pH, redox status, temperature, etc. EPR spin probes are introduced in vivo in low concentrations to reduce interference with the measured physiological function. For this reason, improvement of EPR sensitivity is of crucial importance for EPR spectroscopy, and especially, imaging. We have been developing new spectroscopic and imaging methods to improve accuracy of measured physiological parameters and spatial resolution. Some of these methods are now being incorporated into commercial EPR spectrometers/imagers. Development of new spectroscopic or imaging methods for in vivo applications requires a multi-disciplinary approach. Students with different backgrounds from mathematics and engineering to biology will be able to find a project to work on. For the 2015-2016 year, the focus of my lab will be on building an EPR imager that will be used in subsequent years for pre-clinical studies on mice models. Immediate help is needed to work on the following projects:
- Electromagnetic simulations using Sim4life, http://www.zurichmedtech.com/sim4life/, software to develop bi-modal resonators for EPR Imaging (EPRI). The student is expected to master the software and become an expert in MRI and EPRI resonator designs, and GPU programming. Selected bi-modal models will be 3D-printed and tested on phantoms and in vivo.
- Development of 4D spectral-spatial imaging algorithm that is based on inversion of a large system of linear equations using Tikhonov regularization.