Timothy Eubank, PhD

Professor

Organization:

West Virginia University School of Medicine

Department:

Microbiology, Immunology & Cell Biology

Classification:

Faculty

Member

Organization:

West Virginia University WVU Cancer Institute

Department:

WVU Cancer Institute Research Programs

Classification:

Faculty

Thromboelastography Characterizes the Hypercoagulable State in Murine and Human Breast Cancer. Niemann B, Harvey W, Ranson K, Espinosa C, Hoblitzell HE, Rao P, Lupinacci K, Bailey K, Hazard-Jenkins H, Partin J, Eubank TD, Boone BA. Clin Breast Cancer. 2026 May;26(5):154-161.e1. doi: 10.1016/j.clbc.2026.03.018. Epub 2026 Apr 1.

Neutrophil extracellular trap inhibition revitalizes PDAC immunotherapy responsiveness via reduced fibrosis and TCF1+CD8+ progenitor T-cell expansion. Niemann B, Ivey A, Hopen Q, Dakhlallah D, Brundage K, Mihalik N, Eubank T, Boone BA. Oncoimmunology. 2025 Dec;14(1):2601394. doi: 10.1080/2162402X.2025.2601394. Epub 2025 Dec 18.

Reconfigurable animal bed-EPR resonator assembly for multimodal co-registration. Tseytlin O, Sestito M, Eubank TD, Khramtsov VV, Driesschaert B, Boone BA, Tseytlin M. J Magn Reson. 2026 Jan;382:107995. doi: 10.1016/j.jmr.2025.107995. Epub 2025 Nov 26. PMID: 41319401

Neutrophil Extracellular Traps Modulate Recruitment and Immunosuppression of Macrophages in Pancreatic Adenocarcinoma. Pratt HG, Stevens AM, Sestito M, Ojetunde M, Ivey AD, Mihalik NE, Steinberger KJ, Niemann B, Hoblitzell EH, Wan E, Eubank TD, Boone BA. Cancer Immunol Res. 2025 Oct 1;13(10):1547-1560. doi: 10.1158/2326-6066.CIR-24-0534. PMID: 40742388

Dielectric Signatures of Late Carcinoma Immune Cells Using MMTV-PyMT Mammary Carcinoma Models. Oladokun R, Smith C, Eubank T, Srivastava S. ACS Omega. 2024 Sep 26;9(40):41378-41388. doi: 10.1021/acsomega.4c04210. eCollection 2024 Oct 8. PMID: 39398140

Analysis of single nuclear chromatin accessibility reveals unique myeloid populations in human pancreatic ductal adenocarcinoma. Pratt HG, Ma L, Dziadowicz SA, Ott S, Whalley T, Szomolay B, Eubank TD, Hu G, Boone BA. Clin Transl Med. 2024 Mar;14(3):e1595. doi: 10.1002/ctm2.1595. PMID: 38426634

Transcriptome reprogramming through alternative splicing triggered by apigenin drives cell death in triple-negative breast cancer. Sudhakaran M, Navarrete TG, Mejía-Guerra K, Mukundi E, Eubank TD, Grotewold E, Arango D, Doseff AI. Cell Death Dis. 2023 Dec 13;14(12):824. doi: 10.1038/s41419-023-06342-6. PMID: 38092740

SOX71, A Biocompatible Succinyl Derivative of the Triarylmethyl Radical OX071 for In Vivo Quantitative Oxygen Mapping Using Electron Paramagnetic Resonance. Shaw MA, Poncelet M, Viswakarma N, Vallerini GP, Hameed S, Gluth TD, Geldenhuys WJ, Hoblitzell EH, Eubank TD, Epel B, Kotecha M, Driesschaert B. Mol Imaging Biol. 2024 Jun;26(3):542-552. doi: 10.1007/s11307-023-01869-8. Epub 2023 Nov 9. PMID: 37945971

Dose-Specific Intratumoral GM-CSF Modulates Breast Tumor Oxygenation and Antitumor Immunity. Mihalik NE, Steinberger KJ, Stevens AM, Bobko AA, Hoblitzell EH, Tseytlin O, Akhter H, Dziadowicz SA, Wang L, O'Connell RC, Monaghan KL, Hu G, Mo X, Khramtsov VV, Tseytlin M, Driesschaert B, Wan ECK, Eubank TD. J Immunol. 2023 Nov 15;211(10):1589-1604. doi: 10.4049/jimmunol.2300326. PMID: 37756529

In Vivo Electron Paramagnetic Resonance Molecular Profiling of Tumor Microenvironment upon Tumor Progression to Malignancy in an Animal Model of Breast Cancer. Eubank TD, Bobko AA, Hoblitzell EH, Gencheva M, Driesschaert B, Khramtsov VV. Mol Imaging Biol. 2024 Jun;26(3):424-434. doi: 10.1007/s11307-023-01847-0. Epub 2023 Aug 23. PMID: 37610610

Sexual Dimorphism of Skeletal Muscle in a Mouse Model of Breast Cancer: A Functional and Molecular Analysis. Rentz LE, Whetsell MA, Clayton SA, Mizener AD, Holásková I, Chapa MG, Hoblitzell EH, Eubank TD, Pistilli EE. Int J Mol Sci. 2023 Jul 19;24(14):11669. doi: 10.3390/ijms241411669. PMID: 37511427

The Underexplored Landscape of Hypoxia-Inducible Factor 2 Alpha and Potential Roles in Tumor Macrophages: A Review. Steinberger KJ, Eubank TD. Oxygen (Basel). 2023 Mar;3(1):45-76. doi: 10.3390/oxygen3010005. Epub 2023 Jan 31. PMID: 37124241

Biocompatible Monophosphonated Trityl Spin Probe, HOPE71, for In Vivo Measurement of pO2, pH, and [Pi] by Electron Paramagnetic Resonance Spectroscopy. Gluth TD, Poncelet M, Gencheva M, Hoblitzell EH, Khramtsov VV, Eubank TD, Driesschaert B. Anal Chem. 2023 Jan 17;95(2):946-954. doi: 10.1021/acs.analchem.2c03476. Epub 2022 Dec 20. PMID: 36537829

Multi-Omics Immune Interaction Networks in Lung Cancer Tumorigenesis, Proliferation, and Survival. Ye Q, Hickey J, Summers K, Falatovich B, Gencheva M, Eubank TD, Ivanov AV, Guo NL. Int J Mol Sci. 2022 Nov 29;23(23):14978. doi: 10.3390/ijms232314978. PMID: 36499305

Synthesis and characterization of a biocompatible 13C1 isotopologue of trityl radical OX071 for in vivo EPR viscometry. Poncelet M, Ngendahimana T, Gluth TD, Hoblitzell EH, Eubank TD, Eaton GR, Eaton SS, Driesschaert B. Analyst. 2022 Dec 5;147(24):5643-5648. doi: 10.1039/d2an01527g. PMID: 36373434

Rapid scan EPR: Automated digital resonator control for low-latency data acquisition. O'Connell RC, Tseytlin O, Bobko AA, Eubank TD, Tseytlin M. J Magn Reson. 2022 Dec;345:107308. doi: 10.1016/j.jmr.2022.107308. Epub 2022 Oct 21. PMID: 36356489

Hyperglycemic Oxoaldehyde (Glyoxal)-Induced Vascular Endothelial Cell Damage Through Oxidative Stress Is Protected by Thiol Iron Chelator, Dimercaptosuccinic Acid – Role of Iron in Diabetic Vascular Endothelial Dysfunction. Gurney TO, Oliver PJ, Sliman SM, Yenigalla A, Eubank TD, Nassal DM, Miao J, Zhao J, Hund TJ, Parinandi NL. 2022 Sep 21. In: Parinandi NL, Hund TJ, editors. Cardiovascular Signaling in Health and Disease [Internet]. Cham (CH): Springer; 2022. PMID: 37988529 Free Books & Documents. Review.

 A Multi-Omics Network of a Seven-Gene Prognostic Signature for Non-Small Cell Lung Cancer. Ye Q, Falatovich B, Singh S, Ivanov AV, Eubank TD, Guo NL. Int J Mol Sci. 2021 Dec 25;23(1):219. doi: 10.3390/ijms23010219. PMID: 35008645

Macrophage and Neutrophil Interactions in the Pancreatic Tumor Microenvironment Drive the Pathogenesis of Pancreatic Cancer. Pratt HG, Steinberger KJ, Mihalik NE, Ott S, Whalley T, Szomolay B, Boone BA, Eubank TD. Cancers (Basel). 2021 Dec 31;14(1):194. doi: 10.3390/cancers14010194. PMID: 35008355. Review.

Large-scale synthesis of a monophosphonated tetrathiatriarylmethyl spin probe for concurrent in vivo measurement of pO2, pH and inorganic phosphate by EPR. Gluth TD, Poncelet M, DeVience S, Gencheva M, Hoblitzell EH, Khramtsov VV, Eubank TD, Driesschaert B. RSC Adv. 2021 Jul 27;11(42):25951-25954. doi: 10.1039/d1ra04551b. eCollection 2021. PMID: 34354828

Immune Cell Modulation of the Extracellular Matrix Contributes to the Pathogenesis of Pancreatic Cancer. Ahmad RS, Eubank TD, Lukomski S, Boone BA. Biomolecules. 2021 Jun 17;11(6):901. doi: 10.3390/biom11060901. PMID: 34204306

Formulation and In Vitro Characterization of PLGA/PLGA-PEG Nanoparticles Loaded with Murine Granulocyte-Macrophage Colony-Stimulating Factor. Mihalik NE, Wen S, Driesschaert B, Eubank TD. AAPS PharmSciTech. 2021 Jun 24;22(5):191. doi: 10.1208/s12249-021-02049-z. PMID: 34169366

Biological Applications of Electron Paramagnetic Resonance Viscometry Using a 13C-Labeled Trityl Spin Probe. Velayutham M, Poncelet M, Eubank TD, Driesschaert B, Khramtsov VV. Molecules. 2021 May 8;26(9):2781. doi: 10.3390/molecules26092781. PMID: 34066858

Molecular Analysis of ZNF71 KRAB in Non-Small-Cell Lung Cancer. Ye Q, Mohamed R, Dakhlallah D, Gencheva M, Hu G, Pearce MC, Kolluri SK, Marsh CB, Eubank TD, Ivanov AV, Guo NL. Int J Mol Sci. 2021 Apr 4;22(7):3752. doi: 10.3390/ijms22073752. PMID: 33916522

A Prospective Trial Evaluating the Safety and Systemic Response From the Concurrent Use of Radiation Therapy with Checkpoint Inhibitor Immunotherapy in Metastatic Non-Small Cell Lung Cancer. Mattes MD, Eubank TD, Almubarak M, Wen S, Marano GD, Jacobson GM, Ma PC. Clin Lung Cancer. 2021 Jul;22(4):268-273. doi: 10.1016/j.cllc.2021.01.012. Epub 2021 Jan 25. PMID: 33608212. Clinical Trial.

Cell Competition Boosts Clonal Evolution and Hypoxic Selection in Cancer. Madan E, Peixoto ML, Dimitrion P, Eubank TD, Yekelchyk M, Talukdar S, Fisher PB, Mi QS, Moreno E, Gogna R. Trends Cell Biol. 2020 Dec;30(12):967-978. doi: 10.1016/j.tcb.2020.10.002. Epub 2020 Nov 4. PMID: 33160818 Review.

Hypoxia-Inducible Factor α Subunits Regulate Tie2-Expressing Macrophages That Influence Tumor Oxygen and Perfusion in Murine Breast Cancer. Steinberger KJ, Forget MA, Bobko AA, Mihalik NE, Gencheva M, Roda JM, Cole SL, Mo X, Hoblitzell EH, Evans R, Gross AC, Moldovan L, Marsh CB, Khramstov VV, Eubank TD. J Immunol. 2020 Oct 15;205(8):2301-2311. doi: 10.4049/jimmunol.2000185. Epub 2020 Sep 16. PMID: 32938724

Bone grafting history affects soft tissue healing following implant placement. Kofina V, Demirer M, Erdal BS, Eubank TD, Yildiz VO, Tatakis DN, Leblebicioglu B. J Periodontol. 2021 Feb;92(2):234-243. doi: 10.1002/JPER.19-0709. Epub 2020 Aug 19. PMID: 32779206

Synthesis, Characterization, and Application of a Highly Hydrophilic Triarylmethyl Radical for Biomedical EPR. Sanzhaeva U, Poncelet M, Tseytlin O, Tseytlin M, Gencheva M, Eubank TD, Khramtsov VV, Driesschaert B. J Org Chem. 2020 Aug 21;85(16):10388-10398. doi: 10.1021/acs.joc.0c00557. Epub 2020 Aug 6. PMID: 32698583

Stress-induced Norepinephrine Downregulates CCL2 in Macrophages to Suppress Tumor Growth in a Model of Malignant Melanoma. Steinberger KJ, Bailey MT, Gross AC, Sumner LA, Voorhees JL, Crouser N, Curry JM, Wang Y, DeVries AC, Marsh CB, Glaser R, Yang EV, Eubank TD. Cancer Prev Res (Phila). 2020 Sep;13(9):747-760. doi: 10.1158/1940-6207.CAPR-19-0370. Epub 2020 Jun 9. PMID: 32518084

Macrophage HIF-1α mediates obesity-related adipose tissue dysfunction via interleukin-1 receptor-associated kinase M. Poblete JMS, Ballinger MN, Bao S, Alghothani M, Nevado JB Jr, Eubank TD, Christman JW, Magalang UJ. Am J Physiol Endocrinol Metab. 2020 May 1;318(5):E689-E700. doi: 10.1152/ajpendo.00174.2019. Epub 2020 Mar 10. PMID: 32154744

Wound Fluid Cytokine Profile Following Bone Regeneration Procedures. Leblebicioglu B, Alssum L, Eubank TD, Yildiz VO, Tatakis DN. J Oral Implantol. 2020 Apr 1;46(2):107-113. doi: 10.1563/aaid-joi-D-19-00061. PMID: 31909694

Constitutive AKT Activity Predisposes Lung Fibrosis by Regulating Macrophage, Myofibroblast and Fibrocyte Recruitment and Changes in Autophagy. Dakhlallah D, Wang Y, Bobo TA, Ellis E, Mo X, Piper MG, Eubank TD, Marsh CB. Adv Biosci Biotechnol. 2019 Oct;10(10):346-373. doi: 10.4236/abb.2019.1010027. Epub 2019 Oct 30. PMID: 31750010

Circulating extracellular vesicle content reveals de novo DNA methyltransferase expression as a molecular method to predict septic shock. Dakhlallah DA, Wisler J, Gencheva M, Brown CM, Leatherman ER, Singh K, Brundage K, Karsies T, Dakhlallah A, Witwer KW, Sen CK, Eubank TD, Marsh CB. J Extracell Vesicles. 2019 Sep 28;8(1):1669881. doi: 10.1080/20013078.2019.1669881. eCollection 2019. PMID: 31632618

Development of multifunctional Overhauser-enhanced magnetic resonance imaging for concurrent in vivo mapping of tumor interstitial oxygenation, acidosis and inorganic phosphate concentration. Gorodetskii AA, Eubank TD, Driesschaert B, Poncelet M, Ellis E, Khramtsov VV, Bobko AA. Sci Rep. 2019 Aug 20;9(1):12093. doi: 10.1038/s41598-019-48524-3. PMID: 31431629

Modular imaging system: Rapid scan EPR at 800 MHz. Tseytlin O, Guggilapu P, Bobko AA, AlAhmad H, Xu X, Epel B, O'Connell R, Hoblitzell EH, Eubank TD, Khramtsov VV, Driesschaert B, Kazkaz E, Tseytlin M.

J Magn Reson. 2019 Aug;305:94-103. doi: 10.1016/j.jmr.2019.06.003. Epub 2019 Jun 8. PMID: 31238278

Dextran-conjugated tetrathiatriarylmethyl radicals as biocompatible spin probes for EPR spectroscopy and imaging. Poncelet M, Driesschaert B, Tseytlin O, Tseytlin M, Eubank TD, Khramtsov VV. Bioorg Med Chem Lett. 2019 Jul 15;29(14):1756-1760. doi: 10.1016/j.bmcl.2019.05.017. Epub 2019 May 13. PMID: 31129052

Oxygen-induced leakage of spin polarization in Overhauser-enhanced magnetic resonance imaging: Application for oximetry in tumors. Gorodetskii AA, Eubank TD, Driesschaert B, Poncelet M, Ellis E, Khramtsov VV, Bobko AA. J Magn Reson. 2018 Dec;297:42-50. doi: 10.1016/j.jmr.2018.10.005. Epub 2018 Oct 10. PMID: 30359906

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment. Bobko AA, Eubank TD, Driesschaert B, Khramtsov VV. J Vis Exp. 2018 Mar 16;(133):56624. doi: 10.3791/56624. PMID: 29608148

Gingival Perfusion and Tissue Biomarkers During Early Healing of Postextraction Regenerative Procedures: A Prospective Case Series. Alssum L, Eubank TD, Roy S, Erdal BS, Yildiz VO, Tatakis DN, Leblebicioglu B. J Periodontol. 2017 Nov;88(11):1163-1172. doi: 10.1902/jop.2017.170117. Epub 2017 Jun 23. PMID: 28644107

Interstitial Inorganic Phosphate as a Tumor Microenvironment Marker for Tumor Progression. Bobko AA, Eubank TD, Driesschaert B, Dhimitruka I, Evans J, Mohammad R, Tchekneva EE, Dikov MM, Khramtsov VV. Sci Rep. 2017 Jan 24;7:41233. doi: 10.1038/srep41233. PMID: 28117423

The Effect of Platform Switching on Periimplant Crevicular Fluid Content During Early Wound Healing. Chien HH, Meng HW, Gross AC, Eubank TD, Yildiz VO, Leblebicioglu B. Implant Dent. 2016 Oct;25(5):629-37. doi: 10.1097/ID.0000000000000463. PMID: 27504534 Clinical Trial.

Authors' Response. Escalante MG, Leblebicioglu B, Walters JD, Eubank TD. J Periodontol. 2016 Feb;87(2):101-2. doi: 10.1902/jop.2016.150560. PMID: 26962888 No abstract available.

Poly-arginine conjugated triarylmethyl radical as intracellular spin label. Driesschaert B, Bobko AA, Eubank TD, Samouilov A, Khramtsov VV, Zweier JL. Bioorg Med Chem Lett. 2016 Apr 1;26(7):1742-4. doi: 10.1016/j.bmcl.2016.02.048. Epub 2016 Feb 19. PMID: 26923698

Social stress-enhanced severity of Citrobacter rodentium-induced colitis is CCL2-dependent and attenuated by probiotic Lactobacillus reuteri. Mackos AR, Galley JD, Eubank TD, Easterling RS, Parry NM, Fox JG, Lyte M, Bailey MT. Mucosal Immunol. 2016 Mar;9(2):515-26. doi: 10.1038/mi.2015.81. Epub 2015 Sep 30. PMID: 26422754

New class of 8-aryl-7-deazaguanine cell permeable fluorescent probes. Dhimitruka I, Eubank TD, Gross AC, Khramtsov VV. Bioorg Med Chem Lett. 2015 Oct 15;25(20):4593-6. doi: 10.1016/j.bmcl.2015.08.054. Epub 2015 Aug 21. PMID: 26320620

Detection of nitric oxide production in cell cultures by luciferin-luciferase chemiluminescence. Woldman YY, Eubank TD, Mock AJ, Stevens NC, Varadharaj S, Turco J, Gavrilin MA, Branchini BR, Khramtsov VV. Biochem Biophys Res Commun. 2015 Sep 18;465(2):232-8. doi: 10.1016/j.bbrc.2015.08.001. Epub 2015 Aug 4. PMID: 26253471

Comparison of Azithromycin and Amoxicillin Before Dental Implant Placement: An Exploratory Study of Bioavailability and Resolution of Postoperative Inflammation. Escalante MG, Eubank TD, Leblebicioglu B, Walters JD. J Periodontol. 2015 Nov;86(11):1190-200. doi: 10.1902/jop.2015.150024. Epub 2015 Aug 7. PMID: 26252749 Clinical Trial.

Follistatin-like 3 is a mediator of exercise-driven bone formation and strengthening. Nam J, Perera P, Gordon R, Jeong YH, Blazek AD, Kim DG, Tee BC, Sun Z, Eubank TD, Zhao Y, Lablebecioglu B, Liu S, Litsky A, Weisleder NL, Lee BS, Butterfield T, Schneyer AL, Agarwal S. Bone. 2015 Sep;78:62-70. doi: 10.1016/j.bone.2015.04.038. Epub 2015 Apr 30. PMID: 25937185

Oncolytic HSV virotherapy in murine sarcomas differentially triggers an antitumor T-cell response in the absence of virus permissivity. Leddon JL, Chen CY, Currier MA, Wang PY, Jung FA, Denton NL, Cripe KM, Haworth KB, Arnold MA, Gross AC, Eubank TD, Goins WF, Glorioso JC, Cohen JB, Grandi P, Hildeman DA, Cripe TP. Mol Ther Oncolytics. 2015 Jan 21;1:14010. doi: 10.1038/mto.2014.10. eCollection 2015. PMID: 27119100

Preferential, enhanced breast cancer cell migration on biomimetic electrospun nanofiber 'cell highways'. Nelson MT, Short A, Cole SL, Gross AC, Winter J, Eubank TD, Lannutti JJ. BMC Cancer. 2014 Nov 10;14:825. doi: 10.1186/1471-2407-14-825. PMID: 25385001

Involvement of tumor macrophage HIFs in chemotherapy effectiveness: mathematical modeling of oxygen, pH, and glutathione. Chen D, Bobko AA, Gross AC, Evans R, Marsh CB, Khramtsov VV, Eubank TD, Friedman A. PLoS One. 2014 Oct 8;9(10):e107511. doi: 10.1371/journal.pone.0107511. eCollection 2014. PMID: 25295611

Cytokine concentrations in the cerebrospinal fluid of great danes with cervical spondylomyelopathy. Martin-Vaquero P, da Costa RC, Moore SA, Gross AC, Eubank TD. J Vet Intern Med. 2014 Jul-Aug;28(4):1268-74. doi: 10.1111/jvim.12388. Epub 2014 Jun 25. PMID: 24965833

A sympathetic role for the pancreas in tumor progression? Eubank TD. Brain Behav Immun. 2014 Aug;40:38-9. doi: 10.1016/j.bbi.2014.05.002. Epub 2014 May 14. PMID: 24832547 Free PMC article. No abstract available.

In vivo proton-electron double-resonance imaging of extracellular tumor pH using an advanced nitroxide probe.

Samouilov A, Efimova OV, Bobko AA, Sun Z, Petryakov S, Eubank TD, Trofimov DG, Kirilyuk IA, Grigor'ev IA, Takahashi W, Zweier JL, Khramtsov VV. Anal Chem. 2014 Jan 21;86(2):1045-52. doi: 10.1021/ac402230h. Epub 2014 Jan 6. PMID: 24372284

Intermittent hypoxia exacerbates pancreatic β-cell dysfunction in a mouse model of diabetes mellitus. Sherwani SI, Aldana C, Usmani S, Adin C, Kotha S, Khan M, Eubank T, Scherer PE, Parinandi N, Magalang UJ. Sleep. 2013 Dec 1;36(12):1849-58. doi: 10.5665/sleep.3214. PMID: 24293759

Chronic restraint stress upregulates erythropoiesis through glucocorticoid stimulation. Voorhees JL, Powell ND, Moldovan L, Mo X, Eubank TD, Marsh CB. PLoS One. 2013 Oct 18;8(10):e77935. doi: 10.1371/journal.pone.0077935. eCollection 2013. PMID: 24205034

The impact of adrenergic signaling in skin cancer progression: possible repurposing of β-blockers for treatment of skin cancer. Yang EV, Eubank TD. Cancer Biomark. 2013;13(3):155-60. doi: 10.3233/CBM-130325. PMID: 23912487 Review.

Fcγ receptor-induced soluble vascular endothelial growth factor receptor-1 (VEGFR-1) production inhibits angiogenesis and enhances efficacy of anti-tumor antibodies. Justiniano SE, Elavazhagan S, Fatehchand K, Shah P, Mehta P, Roda JM, Mo X, Cheney C, Hertlein E, Eubank TD, Marsh C, Muthusamy N, Butchar JP, Byrd JC, Tridandapani S. J Biol Chem. 2013 Sep 13;288(37):26800-9. doi: 10.1074/jbc.M113.485185. Epub 2013 Jul 31. PMID: 23902770

Probiotic Lactobacillus reuteri attenuates the stressor-enhanced severity of Citrobacter rodentium infection. Mackos AR, Eubank TD, Parry NM, Bailey MT. Infect Immun. 2013 Sep;81(9):3253-63. doi: 10.1128/IAI.00278-13. Epub 2013 Jun 24. PMID: 23798531

Peri-implant versus periodontal wound healing. Emecen-Huja P, Eubank TD, Shapiro V, Yildiz V, Tatakis DN, Leblebicioglu B. J Clin Periodontol. 2013 Aug;40(8):816-24. doi: 10.1111/jcpe.12127. Epub 2013 Jun 18. PMID: 23772674

Prolonged restraint stress increases IL-6, reduces IL-10, and causes persistent depressive-like behavior that is reversed by recombinant IL-10. Voorhees JL, Tarr AJ, Wohleb ES, Godbout JP, Mo X, Sheridan JF, Eubank TD, Marsh CB. PLoS One. 2013;8(3):e58488. doi: 10.1371/journal.pone.0058488. Epub 2013 Mar 8. PMID: 23520517

Phosphonated trityl probes for concurrent in vivo tissue oxygen and pH monitoring using electron paramagnetic resonance-based techniques. Dhimitruka I, Bobko AA, Eubank TD, Komarov DA, Khramtsov VV. J Am Chem Soc. 2013 Apr 17;135(15):5904-10. doi: 10.1021/ja401572r. Epub 2013 Apr 5. PMID: 23517077

Pancreatic cancer-associated stellate cells promote differentiation of myeloid-derived suppressor cells in a STAT3-dependent manner. Mace TA, Ameen Z, Collins A, Wojcik S, Mair M, Young GS, Fuchs JR, Eubank TD, Frankel WL, Bekaii-Saab T, Bloomston M, Lesinski GB. Cancer Res. 2013 May 15;73(10):3007-18. doi: 10.1158/0008-5472.CAN-12-4601. Epub 2013 Mar 20. PMID: 23514705

Macrophage microvesicles induce macrophage differentiation and miR-223 transfer. Ismail N, Wang Y, Dakhlallah D, Moldovan L, Agarwal K, Batte K, Shah P, Wisler J, Eubank TD, Tridandapani S, Paulaitis ME, Piper MG, Marsh CB. Blood. 2013 Feb 7;121(6):984-95. doi: 10.1182/blood-2011-08-374793. Epub 2012 Nov 9. PMID: 23144169

Hypoxia inducible factors-mediated inhibition of cancer by GM-CSF: a mathematical model. Chen D, Roda JM, Marsh CB, Eubank TD, Friedman A. Bull Math Biol. 2012 Nov;74(11):2752-77. doi: 10.1007/s11538-012-9776-3. Epub 2012 Oct 17. PMID: 23073704

Stabilization of HIF-2α induces sVEGFR-1 production from tumor-associated macrophages and decreases tumor growth in a murine melanoma model. Roda JM, Wang Y, Sumner LA, Phillips GS, Marsh CB, Eubank TD. J Immunol. 2012 Sep 15;189(6):3168-77. doi: 10.4049/jimmunol.1103817. Epub 2012 Aug 6. PMID: 22869907

Modeling the inhibition of breast cancer growth by GM-CSF. Szomolay B, Eubank TD, Roberts RD, Marsh CB, Friedman A. J Theor Biol. 2012 Jun 21;303:141-51. doi: 10.1016/j.jtbi.2012.03.024. Epub 2012 Mar 28. PMID: 22763136

In vivo monitoring of pH, redox status, and glutathione using L-band EPR for assessment of therapeutic effectiveness in solid tumors. Bobko AA, Eubank TD, Voorhees JL, Efimova OV, Kirilyuk IA, Petryakov S, Trofimiov DG, Marsh CB, Zweier JL, Grigor'ev IA, Samouilov A, Khramtsov VV. Magn Reson Med. 2012 Jun;67(6):1827-36. doi: 10.1002/mrm.23196. Epub 2011 Nov 23. PMID: 22113626

HIFs: a-cute answer to inflammation? Eubank TD, Marsh CB. Blood. 2011 Jul 21;118(3):485-7. doi: 10.1182/blood-2011-05-355164. PMID: 21778347 Free article. No abstract available.

Hypoxia-inducible factor-2α regulates GM-CSF-derived soluble vascular endothelial growth factor receptor 1 production from macrophages and inhibits tumor growth and angiogenesis. Roda JM, Sumner LA, Evans R, Phillips GS, Marsh CB, Eubank TD. J Immunol. 2011 Aug 15;187(4):1970-6. doi: 10.4049/jimmunol.1100841. Epub 2011 Jul 15. PMID: 21765015

LyGDI, a novel SHIP-interacting protein, is a negative regulator of FcγR-mediated phagocytosis. Mehta P, Wavreille AS, Justiniano SE, Marsh RL, Yu J, Burry RW, Jarjoura D, Eubank T, Caligiuri MA, Butchar JP, Tridandapani S. PLoS One. 2011;6(6):e21175. doi: 10.1371/journal.pone.0021175. Epub 2011 Jun 14. PMID: 21695085

Opposing roles for HIF-1α and HIF-2α in the regulation of angiogenesis by mononuclear phagocytes. Eubank TD, Roda JM, Liu H, O'Neil T, Marsh CB. Blood. 2011 Jan 6;117(1):323-32. doi: 10.1182/blood-2010-01-261792. Epub 2010 Oct 15. PMID: 20952691

Azithromycin decreases crevicular fluid volume and mediator content. Ho W, Eubank T, Leblebicioglu B, Marsh C, Walters J. J Dent Res. 2010 Aug;89(8):831-5. doi: 10.1177/0022034510368650. Epub 2010 Apr 16. PMID: 20400725

Monocytes and macrophages regulate immunity through dynamic networks of survival and cell death. Parihar A, Eubank TD, Doseff AI. J Innate Immun. 2010;2(3):204-15. doi: 10.1159/000296507. Epub 2010 Mar 16. PMID: 20375558 Review.

Hyperglycemic oxoaldehyde, glyoxal, causes barrier dysfunction, cytoskeletal alterations, and inhibition of angiogenesis in vascular endothelial cells: aminoguanidine protection. Sliman SM, Eubank TD, Kotha SR, Kuppusamy ML, Sherwani SI, Butler ES, Kuppusamy P, Roy S, Marsh CB, Stern DM, Parinandi NL. Mol Cell Biochem. 2010 Jan;333(1-2):9-26. doi: 10.1007/s11010-009-0199-x. Epub 2009 Jul 8. PMID: 19585224

Trityl-based EPR probe with enhanced sensitivity to oxygen. Bobko AA, Dhimitruka I, Eubank TD, Marsh CB, Zweier JL, Khramtsov VV. Free Radic Biol Med. 2009 Sep 1;47(5):654-8. doi: 10.1016/j.freeradbiomed.2009.06.007. Epub 2009 Jun 10. PMID: 19523513

Survival of monocytes and macrophages and their role in health and disease. Hunter M, Wang Y, Eubank T, Baran C, Nana-Sinkam P, Marsh C. Front Biosci (Landmark Ed). 2009 Jan 1;14(11):4079-102. doi: 10.2741/3514. PMID: 19273336 Review.

Granulocyte macrophage colony-stimulating factor inhibits breast cancer growth and metastasis by invoking an anti-angiogenic program in tumor-educated macrophages. Eubank TD, Roberts RD, Khan M, Curry JM, Nuovo GJ, Kuppusamy P, Marsh CB. Cancer Res. 2009 Mar 1;69(5):2133-40. doi: 10.1158/0008-5472.CAN-08-1405. Epub 2009 Feb 17. PMID: 19223554

M-CSF signals through the MAPK/ERK pathway via Sp1 to induce VEGF production and induces angiogenesis in vivo. Curry JM, Eubank TD, Roberts RD, Wang Y, Pore N, Maity A, Marsh CB. PLoS One. 2008;3(10):e3405. doi: 10.1371/journal.pone.0003405. Epub 2008 Oct 14. PMID: 18852899

Latency associated peptide has in vitro and in vivo immune effects independent of TGF-beta1. Ali NA, Gaughan AA, Orosz CG, Baran CP, McMaken S, Wang Y, Eubank TD, Hunter M, Lichtenberger FJ, Flavahan NA, Lawler J, Marsh CB. PLoS One. 2008 Apr 2;3(4):e1914. doi: 10.1371/journal.pone.0001914. PMID: 18392110

The role of inflammation in the pathogenesis of idiopathic pulmonary fibrosis. Bringardner BD, Baran CP, Eubank TD, Marsh CB. Antioxid Redox Signal. 2008 Feb;10(2):287-301. doi: 10.1089/ars.2007.1897. PMID: 17961066 Review.

Nitroaspirin (NCX-4016), an NO donor, is antiangiogenic through induction of loss of redox-dependent viability and cytoskeletal reorganization in endothelial cells. Parinandi NL, Sharma A, Eubank TD, Kaufman BF, Kutala VK, Marsh CB, Ignarro LJ, Kuppusamy P. Antioxid Redox Signal. 2007 Nov;9(11):1837-49. doi: 10.1089/ars.2007.1603. PMID: 17760507

Norepinephrine up-regulates the expression of vascular endothelial growth factor, matrix metalloproteinase (MMP)-2, and MMP-9 in nasopharyngeal carcinoma tumor cells. Yang EV, Sood AK, Chen M, Li Y, Eubank TD, Marsh CB, Jewell S, Flavahan NA, Morrison C, Yeh PE, Lemeshow S, Glaser R. Cancer Res. 2006 Nov 1;66(21):10357-64. doi: 10.1158/0008-5472.CAN-06-2496. PMID: 17079456

The role of the NADPH oxidase complex, p38 MAPK, and Akt in regulating human monocyte/macrophage survival. Wang Y, Zeigler MM, Lam GK, Hunter MG, Eubank TD, Khramtsov VV, Tridandapani S, Sen CK, Marsh CB. Am J Respir Cell Mol Biol. 2007 Jan;36(1):68-77. doi: 10.1165/rcmb.2006-0165OC. Epub 2006 Aug 24. PMID: 16931806

GM-CSF induces expression of soluble VEGF receptor-1 from human monocytes and inhibits angiogenesis in mice. Eubank TD, Roberts R, Galloway M, Wang Y, Cohn DE, Marsh CB. Immunity. 2004 Dec;21(6):831-42. doi: 10.1016/j.immuni.2004.10.011. PMID: 15589171

M-CSF induces vascular endothelial growth factor production and angiogenic activity from human monocytes. Eubank TD, Galloway M, Montague CM, Waldman WJ, Marsh CB. J Immunol. 2003 Sep 1;171(5):2637-43. doi: 10.4049/jimmunol.171.5.2637. PMID: 12928417

Inhibition of bacterial RNase P by aminoglycoside-arginine conjugates. Eubank TD, Biswas R, Jovanovic M, Litovchick A, Lapidot A, Gopalan V. FEBS Lett. 2002 Jan 30;511(1-3):107-12. doi: 10.1016/s0014-5793(01)03322-1. PMID: 11821058

Timothy D. Eubank, PhD, is a Professor in the Department of Microbiology, Immunology and Cell Biology in the WVU School of Medicine. His research program focuses on how myeloid cells - particularly circulating monocytes and tumor-associated macrophages (TAMs) - regulate vascular dysfunction, hypoxia, immune suppression, and therapeutic resistance in solid tumors.

Dr. Eubank’s laboratory has made important contributions to understanding how macrophages can be therapeutically reprogrammed within the tumor microenvironment. His group demonstrated that GM-CSF induces monocytes and macrophages to produce soluble VEGFR-1, which sequesters excessive VEGF and promotes an angiostatic phenotype. His laboratory also helped define distinct roles for HIF-1α and HIF-2α in tumor macrophages and has continued to investigate how macrophage HIF biology influences tumor vascular function, oxygenation, and immune organization.

Current work in the Eubank laboratory is centered on pancreatic ductal adenocarcinoma, a highly hypoxic and immunologically resistant cancer. The laboratory is investigating how hypoxia disrupts the relationship between tumor antigen processing and MHC-mediated antigen presentation, thereby limiting effective T cell recognition. A complementary area of research examines whether macrophage-directed vascular and oxygen remodeling can improve immune cell organization and increase tumor responsiveness to immunotherapy. The laboratory also studies circulating monocyte subsets as potential biomarkers and functional mediators of chemotherapy response, including their recruitment and differentiation within pancreatic tumors.

These studies integrate genetically engineered orthotopic PDAC tumor models, human biospecimens, electron paramagnetic resonance oxygen imaging, multiplex fluorescence imaging, spatial transcriptomics, single cell analysis, flow cytometry, and computational approaches.

Dr. Eubank has published extensively in tumor immunology, macrophage biology, angiogenesis, and hypoxia and has received several research grants including PI on the K99/R00 Pathway to Independence Award, and multiple R01s from the National Cancer Institute and other federal and foundation sources. He is also deeply committed to mentoring graduate students and junior faculty, with particular emphasis on scientific rigor, grant writing, career development, and collaborative research.

 

Immunology and Microbial Pathogenesis (IMP) Program

Dr. Eubank’s research focuses on how monocytes and macrophages regulate vascular dysfunction, hypoxia, immune suppression, and therapeutic resistance in solid tumors, with particular emphasis on pancreatic ductal adenocarcinoma and breast cancer. His laboratory investigates functionally distinct myeloid cell populations that promote tumor progression, sustain abnormal and unproductive angiogenesis, and contribute to the development of chronically hypoxic tumor microenvironments.

A major component of this work is the quantitative measurement of tumor microenvironment physiology. In collaboration with investigators in biochemistry and pharmaceutical sciences, including Drs. Valery Khramtsov, Benoit Driesschaert, and Mark Tseytlin, the laboratory uses particulate and soluble electron paramagnetic resonance probes and EPR imaging to measure tumor oxygenation and related physiological parameters. These approaches can be integrated with multiplex immunofluorescence, immunohistochemistry, spatial analysis, and complementary imaging methods to define how myeloid cell behavior is associated with regional hypoxia, vascular function, and immune organization.

The laboratory is also investigating how hypoxia alters tumor antigen processing and MHC-mediated presentation, thereby limiting effective T cell recognition in pancreatic cancer. Complementary studies examine whether macrophage-directed reprogramming, particularly through induction of soluble VEGFR-1 and sequestration of excessive VEGF, can improve vascular function, remodel tumor oxygenation, and increase responsiveness to immunotherapy.

An additional area of interest is the biology of circulating monocyte subsets as both biomarkers and mediators of treatment response. These studies seek to determine how distinct monocyte populations are recruited into pancreatic tumors, how they differentiate within the tumor microenvironment, and whether their abundance or functional state can predict therapeutic outcome. This work includes collaboration with robotic surgical oncologist, Dr. Brian Boone.