Stacey E Anderson , PhD

Team Leader, Immunotoxicology and Hazard Identification laboratory, Allergen and Clinical Immunology Branch (ACIB), Health Effects Laboratory Division, National Institute for Occupational Safety and Health (NIOSH)

Adjunct Assistant Professor

Organization:

West Virginia University School of Medicine

Department:

Microbiology, Immunology & Cell Biology

Classification:

Adjunct Faculty

• BA, West Virginia University, 1998
• PhD, West Virginia University, 2004

Anderson SE, Karandikar U, Mason N , Trott RL , Bishop CP , Bidwai AP [2003]. The Drosophila SSL Gene Is Expressed In Larvae, Pupae, and Adults, Exhibits Sexual Dimorphism, and Mimics Properties of the Beta Subunit of Casein Kinase II. 301(4):941-947

Anderson SE, Wells JR, Fedorowicz A, Butterworth LF, Meade BJ, Munson AE [2007]. Evaulation of the Contact and Respiratory Sensitization Potential of Volatile Organic Compounds Generated by Simulated Indoor Air Chemistry. Toxicol Sci 97(2):355-363.

Fairley KJ, Purdy R, Kearns S, Anderson SE, Meade BJ [2007]. Exposure to the Immunosuppressant, Perflourooctanonoic Acid, Enhances the Murine IgE and Airway Hyperreactivity Response to Ovalbumin. Toxicol Sci 97(2):375-383.

Anderson SE, Meade BJ, Butterworth LF, Munson AE [2007]. The Humoral Immune Response of Mice Exposed to Manual Metal Arc-Stainless Steel Welding Fumes. J Immunot 4(1):15-23.

Blachere FM, Lindsley WG, Slaven JE, Green BJ, Anderson SE, Chen BT, Beezhold D [2007]. Bioaerosol Sampling for the Detection of Aerosolized Influenza Virus. Influenza Other Respir Viruses 1(3):113-120.

Myers LP, Law BF, Fedorowicz A, Sussman G, Shapiro M, Siegel PD, Butterworth LF,Anderson SE, Meade BJ, Beezhold D [2007]. Identification of phenolic dermal sensitizers in a wound closure tape. J Immunot 4:303-310.

Anderson SE, Ham J, Munson AE [2008]. Irritancy and Sensitization Potential of Glyoxylic Acid. J Immunot 5(2):93-98.

Anderson SE, Diotte NM, Munson AE, Tomblyn S, Meade BJ [2008]. The Humoral Immune Response of Mice Exposed to Simulated Road Paving-Like Asphalt Fumes. J Immunot. 5(3); 307-13.

DeWitt JC, Shnyra A, Badr MZ, Loveless SE, Hoban D, Frame SR, Cunard R, Anderson SE, Meade BJ, Luebke RW, Luster M [2008]. Immunotoxicity of PFOA and other putative PPAR? ligands. Crit Rev Toxicol. 2(1).

Anderson SE, Brown K, Butterworth L, Fedorowicz A, Jackson LG, Frasch H, Beezhold D, Munson AE, Meade BJ [2008]. Evaluation of Irritancy and Sensitization Potential of Metalworking Fluids and Components. Immunot 6(1):19-29.

Blachere F, Lindsley WG, Pearce TA, Anderson SE, Fisher M, Khakoo R, Meade BJ, Lander O, Davis S, Thewlis RE, Celik I, Chen TB, Beezhold D [2009]. Measurement of Airborne Influenza in a Hospital Emergency Department. Clin Infect Dis. 48.

Siegel PD, Fedorowicz A, Butterworth L, Law BF, Anderson SE, Snyder JA, Beezhold D.Physical-chemical and solvent considerations in evaluating the influence of carbon chain length on the skin sensitization activity of 1-bromoalkanes. Toxicol Sci 107(1):78-84.

Chipinda I, Anderson SE, Butterworth L, Beezhold D, Siegel PD [2008]. Increased Cell Proliferation in Spleen and Lymph Nodes Peripheral to Contact Allergen Application Site. Toxicology 257(3):113-116.

Anderson SE, Munson AE, Butterworth L, Germolec D, Morgan DL, Roycroft JA, Dill J, Meade BJ [2010]. Whole Body Inhalation Exposure to 1-Bromopropane Suppresses the IgM Response to Sheep Red Blood Cells in Female B6C3F1 Mice and Fisher 344/N Rats. Inhal Toxicol. 2010 Feb;22(2):125-32.

Anderson SE, Brown K, Butterworth L, Fedorowicz A, Jackson LG, Frasch HF, Beezhold D, Munson AE, Meade BJ [2009]. Evaluation of Irritancy and Sensitization Potential of Metalworking Fluid Mixtures and Components. J Immunot 6(1):19-29.

Frasch H, Zang L, Barbero A, Anderson SE [2010]. In vitro dermal penetration of 4-chloro-3-methylphenol from commercial metalworking fluid and aqueous vehicl. 73(20):1394-1405

Anderson SE, Jackson LG, Franko J, Wells JR [2010]. Evaluation of Dicarbonyls Generated in a Simulated Indoor Air Environment Using an in Vitro Exposure System. Toxicol Sci 115(2):453-461

Anderson SE, Umbright CM, Sellamuthu R, Fluharty K, Kashon ML, Franko J, Jackson LG, Johnson V, Joseph P [2010]. Irritancy and Allergic Responses Induced by Topical Application of Ortho-Phthalaldehyde. Toxicol Sci 115(2):435-443

Anderson SE, Siegel PD, Meade BJ [2011]. The LLNA: A Brief Review of Recent Advances and Limitations. J Allergy 2011:424403.

Franko J, Jackson LG, Meade BJ, Anderson SE [2011]. Allergic Potential and Immunotoxicity Induced by Topical Application of 1-Chloro-4-(trifluoromethyl)benzene (PCBTF) in a Murine Model [2011]. Allergy:238513.

Franko J, Meade BJ, Frasch H, Barbero A, Anderson SE [2012]. Dermal Penetration Potential of Perfluorooctanoic Acid (PFOA) in Human and Mouse Skin. J Toxicol Environ Health 75(1):50-62.

Anderson SE, Franko J, Lukomska E, Meade BJ [2011]. Potential immunotoxicological health effects following exposure to COREXIT 9500A during clean-up of the Deep Water Horizon Oil Spill. J Toxicol Environ Health 74(21):1419-1430.

Franko J, Jackson LG, Hubbs AF, Kashon ML, Meade BJ, Anderson SE [2012]. Evaluation of furfuryl alcohol sensitization potential following dermal and pulmonary exposure: Enhancement of airway responsiveness. Toxicol Sci 125(1):105-115.

View more at PubMed

Current Projects

Evaluation of Immunotoxic Chemicals

The main focus of my lab is to evaluate the immunotoxicity caused by exposure to occupationally relevant chemicals using a standardized sequence of immunotoxicological assays which will evaluate the effects of these chemicals on the immune system. The test articles/chemicals we evaluate are identified through collaborations with members of industry, other divisions of NIOSH and other branch members within the Health Effects Laboratory Division of NIOSH, and the National Institute of Environmental Health Sciences (NIEHS). Through these collaborations, sensitizing components and chemicals present in manufactured goods including: metal working fluids, thermal transfer ribbon and wound closure bandages have been identified using the local lymph node assay (LLNA) (Myers et al. 2007; Anderson et al. 2009a); immunotoxicological evaluations of welding and asphalt fumes have been conducted (Anderson 2008; Anderson 2007) and the adverse health effects after exposure to other high production volume chemicals such as 1-bromopropane (1-BP) have been characterized in the laboratory (Anderson et al. 2009b). In addition, the lab also has experience utilizing a murine model of asthma in the evaluation of chemicals contributing to occupational asthma (Fairley et al. 2007).

Investigations into Health Effects caused by exposure to indoor air reaction products

Other research projects and interests include the identification, quantification and evaluation of immunotoxicity of the reaction products of gas-phase compounds, especially dicarbonyls, present in the indoor environment using a novel in vitro exposure system that utilizes the air/cell interphase with hopes to identify biomarkers that can be used to screen indoor environments. This project is a synergistic approach combining toxicology and indoor chemistry. Past successes include hazard identification of many of these indoor air reaction products as irritants and sensitizers and investigations into changes in gene expression after exposure to the indoor air reaction products.

The Contribution of Chemical Adjuvancy to Occupational Asthma

During the past several decades there has been a remarkable and unexplained increase in the prevalence of asthma. While the hygiene hypothesis provides one potential explanation, individuals in industrial societies are exposed to an increasing number of chemicals. While many chemicals are known to directly induce asthma, there is the potential for chemicals to function as adjuvants, augmenting the immune response induced by other chemical and protein allergens. Recent studies in our lab have demonstrated the potential of several non-allergenic chemicals including Bisphenol A (BPA), triclosan, and perflourooctanonic acid (PFOA), to act as adjuvants, augmenting the IgE and airway hyper-reactivity responses to the protein, ovalbumin. Currently the EPA’s harmonized test guidelines for Immunotoxicity testing do not require a test for adjuvancy and no validated assays are available for this purpose. Therefore, understanding the mechanism(s) by which chemicals enhance allergic-like responses is important to aid in the development of more effective methods to screen for chemical adjuvancy.

Curriculum Vitae

Education

2004-2007
Post-Doctoral (Immunotoxicology), National Institute of Occupational Safety and Health (NIOSH), Health Effects Laboratory Division (HELD), Allergen and Clinical Immunology Branch (ACIB), Morgantown, WV

2004
Ph.D., Biology (Cellular and Molecular) West Virginia University, Morgantown, WV

1998
B.A., Biology West Virginia University, Morgantown, WV

Professional Experience

2007-present
Team Leader, Immunotoxicology and Hazard Identification laboratory, Allergen and Clinical Immunology Branch (ACIB), Health Effects Laboratory Division

2007-2010
Society of Toxicology (SOT) Immunotoxicology Specialty Section Post-doctoral Representative

2007-present
NORA Immune and Dermal Sector program Assistant Coordinator

2008-present
SOT World Wide Web Task Force

2009-present
NIOSH/HELD Representative for the Radiation Safety Committee

2011-present
Animal Care and Use Committee Member

2011-present
Adjunct Assistant Professor, Department of Microbiology, Immunology and Cell Biology, West Virginia University

Honors

NIOSH Bullard-Sherwood award (2008)

Charles C. Shepard Science Award Nominee (2010)

Book Chapters

Anderson SE, Munson AE, Meade BJ [2006]. Analysis of Immunotoxicity by Enumeration of Antibody-Producing B Cells. In: Current Protocols in Toxicology. Vol. 2. New York, NY: John Wiley & Sons, Inc., pp. 1-19.

Research Techniques

Whole body plethysmography
Plethysmography in rodent models using Buxco Research Systems. 

Cell culture and isolation
A549 cultures 3H-Thymidine incorporation based proliferation assays.

FACS-based skills
Multi-color flow cytometry analysis Familiar with FACSVantage and LSRII models.

Molecular Biology
Real time PCR, PCR, RT-PCR, gel electrophoresis, Drosophila genetics (P-element insertions, ? mutagenesis), Southern Blot, RNA/DNA isolations, DNA microarray, Primer design, Genomic libraries, Restriction enzyme digests, Plasmid Rescue, ELISA

Computing
FlowJo, GraphPad, EndNote, Microsoft Office

Animals
Small rodent exposure (mouse and rat), small rodent necropsy (mouse and rat), intravenous and intraperitoneal injections, pulmonary aspirations, tail bleeds