Kathy Brundage, PhD

Director, WVU Flow Cytometry & Single Cell Core Facility
Professor

Organization:

West Virginia University School of Medicine

Department:

Microbiology, Immunology & Cell Biology

Classification:

Faculty

Member

Organization:

West Virginia University WVU Cancer Institute

Department:

WVU Cancer Institute Research Programs

Classification:

Staff

• BS, Quinnipiac University, 1985
• PhD, University of Pennsylvania, 1994

Cell Sorter Cleaning Practices and Their Impact on Instrument Sterility.
Box A, Holmes L, DeLay M, Adams D, Bergeron A, Clise-Dwyer K, Cochran M, Guerra RDR, Meyer EM, Brundage KM. J Biomol Tech. 2022 Feb 2;33(1):3fc1f5fe.e2675d74. doi: 10.7171/3fc1f5fe.e2675d74. eCollection 2022 Apr 15.

The impact of opioid exposure during pregnancy on the human neonatal immune profile.
Miller NW, Seman BG, Akers SM, Povroznik JM, Brundage K, Fang W, Robinson CM.Pediatr Res. 2022 Dec;92(6):1566-1574. doi: 10.1038/s41390-022-02014-x. Epub 2022 Mar 14.

Myeloid-Derived Suppressor Cells Gain Suppressive Function during Neonatal Bacterial Sepsis.
Vance JK, Rawson TW, Povroznik JM, Brundage KM, Robinson CM.Int J Mol Sci. 2021 Jun 30;22(13):7047. doi: 10.3390/ijms22137047.

 Evaluating the Effects of Cell Sorting on Gene Expression.
Box A, DeLay M, Tighe S, Chittur SV, Bergeron A, Cochran M, Lopez P, Meyer EM, Saluk A, Thornton S, Brundage K.J Biomol Tech. 2020 Sep;31(3):100-111. doi: 10.7171/jbt.20-3103-004.

Opening large-conductance potassium channels selectively induced cell death of triple-negative breast cancer.
Sizemore G, McLaughlin S, Newman M, Brundage K, Ammer A, Martin K, Pugacheva E, Coad J, Mattes MD, Yu HG.BMC Cancer. 2020 Jun 26;20(1):595. doi: 10.1186/s12885-020-07071-1.

 

I am the Director of the WVU Flow Cytometry & Single Cell Core Facility (FCSCCF), formerly the WVU Flow Cytometry Core Facility (FCCF).  As the Director of the Facility, I am responsible for the day-to-day operations of the facility, supervising and training the facility staff, user training, writing grants, and billing.  I regularly consult with investigators from across the WVU campus who wish to use the facility on experimental design, reagent purchases and data analysis.  I have created many SOPs to cover the work performed in the facility as well as a training manual on the basics of flow cytometry that is given to new users of the facility.  Through a quarterly newsletter and seminars, I keep Facility users abreast of the latest techniques and technologies.  Finally, I represent the interests of the WVU FCSCCF users to the administration and develop practical management policies for the facility.

Since becoming Director (appointed in 2007), I have expanded the facility to include additional flow cytometers and new technologies.  This expansion included the purchasing of a full spectrum 3 laser Cytek Aurora (purchased with an NIH S10 Equipment Grant 0D028605), the Malvern Nanosight NS300 for submicron particle detection, sizing and fluorescence, the MSD (Meso Scale Discovery) system for multiplexing detection of proteins in fluids and cell extracts, the 10x Genomics Chromium which expanded the single cell analysis capabilities of the core, and the 10x Genomics CytAssist for spatial transcriptomics.  I am a member of ISAC’s Shared Resources Laboratories (SRL) Committee that has developed on a certification program for Flow Cytometry Core Facilities.  In addition, I am a member of ISAC’s Education Committee and coordinator of ISAC’s Educational Webinar series.  I am a member of the Association of Biomedical Resources Facility (ABRF).  Within ABRF, I am a member and treasurer of the ABRF Chapter, Mid-Atlantic Directors, and Staff of Scientific Cores (MAD SSCI) as well as a member of the editorial board of the society’s journal, Journal of Biomolecular Techniques. 

In addition, to my responsibilities in the WVU FCSCCF, I am coordinator for the IMMB undergraduate research program.  In this role, I meet with IMMB students who are interested in obtaining experience is an individual research lab or a core facility, discuss their scientific interests, and future career goals. Based on our discussion, I assist them in finding a lab/facility to work in.

 

Curriculum Vitae

Education

1985
BS in Medical Technology from Quinnipiac University in Hamden, CT

1994
PhD in Immunology from University of Pennsylvania in Philadelphia, PA

1994-1999
Post-doctoral Fellow at Thomas Jefferson Medical College in Philadelphia, PA

1999-2001
Post-doctoral Fellow at West Virginia University in Morgantown, WV

Grant Support

P20 RR032138 CoBRE for Signal Transduction and Cancer Phase III
Funding period: 07/15/11-07/14/16
Role: Co-I Flow Cytometry Core Technical Director
The goals of this project are to nurture collaborative interactions, support core facilities, and mentor junior scientists involved in studies of signal transduction in cancer.

Presentations

May 2010. WVU flow cytometry core facility. Obsorne Research Group of MBRCC. Morgantown, WV

August, 2011. WVU flow cytometry core facility. 2nd Annual Alexander B. Obsorne Hematopoietic Malignancy and Transplantation Program Retreat. Morgantown, WV

Current Appointments

7/2015 – present

Director – WVU Flow Cytometry & Single Cell Core Facility

7/2022 – present

Professor (Service Track) – Dept. Micro/Immuno/Cell Bio

1/2010 – present

Associate Member – WVU Cancer Institute

Non-Programmatically Aligned Cancer Center Member