- PhD, Peking University, 2006
Du J, Yanagida A, Knight K, Engel AL, Vo AH, Jankowski C, Sadilek M, Tran VT, Manson MA, Ramakrishnan A, Hurley JB, Chao JR. "Reductive carboxylation is a major metabolic pathway in the retinal pigment epithelium." Proc Natl Acad Sci U S A. 2016 Dec 1. pii: 201604572. [Epub ahead of print]
Gu H, Carroll PA, Du J, Zhu J, Neto FC, Eisenman RN, Raftery D. "Quantitative Method to Investigate the Balance between Metabolism and Proteome Biomass: Starting from Glycine." Angew Chem Int Ed Engl. 2016 Dec 12;55(50):15646-15650. doi: 10.1002/anie.201609236.
Zhang L, Du J, Justus S, Hsu CW, Bonet-Ponce L, Wu WH, Tsai YT, Wu WP, Jia Y, Duong JK, Mahajan VB, Lin CS, Wang S, Hurley JB, Tsang SH. "Reprogramming metabolism by targeting sirtuin 6 attenuates retinal degeneration." J Clin Invest. 2016 Dec 1;126(12):4659-4673. doi: 10.1172/JCI86905.
Contreras L, Ramirez L, Du J, Hurley JB, Satrústegui J, de la Villa P. "Deficient glucose and glutamine metabolism in Aralar/AGC1/Slc25a12 knockout mice contributes to altered visual function." Mol Vis. 2016 Oct 12;22:1198-1212.
Wu HE, Baumgardt SL, Fang J, Paterson M, Liu Y, Du J, Shi Y, Qiao S, Bosnjak ZJ, Warltier DC, Kersten JR, Ge ZD. "Cardiomyocyte GTP Cyclohydrolase 1 Protects the Heart Against Diabetic Cardiomyopathy." Sci Rep. 2016 Jun 13;6:27925. doi: 10.1038/srep27925.
Du J, Rountree A, Cleghorn WM, Contreras L, Lindsay KJ, Sadilek M, Gu H, Djukovic D, Raftery D, Satrústegui J, Kanow M, Chan L, Tsang SH, Sweet IR, Hurley JB. "Phototransduction Influences Metabolic Flux and Nucleotide Metabolism in Mouse Retina." J Biol Chem. 2016 Feb 26;291(9):4698-710. doi: 10.1074/jbc.M115.698985.
Du J, Linton JD, Hurley JB. "Probing Metabolism in the Intact Retina Using Stable Isotope Tracers." Methods Enzymol. 2015;561:149-70. doi: 10.1016/bs.mie.2015.04.002. Review.
Hurley JB, Lindsay KJ, Du J. "Glucose, lactate, and shuttling of metabolites in vertebrate retinas." J Neurosci Res. 2015 Jul;93(7):1079-92. doi: 10.1002/jnr.23583. Review.
Gu H, Du J, Carnevale Neto F, Carroll PA, Turner SJ, Chiorean EG, Eisenman RN, Raftery D. "Metabolomics method to comprehensively analyze amino acids in different domains." Analyst. 2015 Apr 21;140(8):2726-34. doi: 10.1039/c4an02386b.
Carroll PA, Diolaiti D, McFerrin L, Gu H, Djukovic D, Du J, Cheng PF, Anderson S, Ulrich M, Hurley JB, Raftery D, Ayer DE, Eisenman RN. "Deregulated Myc requires MondoA/Mlx for metabolic reprogramming and tumorigenesis." Cancer Cell. 2015 Feb 9;27(2):271-85. doi: 10.1016/j.ccell.2014.11.024.
Metabolic signaling in healthy and diseased retina, metabolomics, patient-derived stem cells.
Description of Research
Metabolic network in the visual system
The eye consumes extremely high energy every second and the disturbance of its metabolism can cause blindness. Understanding its unique metabolism is fundamental for retinal degenerative diseases like inherited degeneration, age related macular degeneration (AMD) and diabetic retinopathy.
We are addressing several questions:
- How are nutrients transported and utilized in retinal cells?
- How do photoreceptor cells,glial cells and retinal pigment epithelium coordinate together to make metabolic circuits to maintain their specialized functions?
- How important are glycolysis, mitochondrial oxidative phosphorylation and reductive carboxylation in the retina?
We are using targeted metabolomics. metabolic flux analysis and cell-specific conditional knockout mice animals to test our models.
Targeting metabolism to fight blindness
Our goal is to reveal the metabolic basis for retinal degenerative diseases and to develop new approaches to slow down or prevent blindness. We are using human primary cultured cells, patient-derived retinal cells by iPS technology, genetically modified mouse.models and collaborating with clinicians to test our hypotheses with both targeted and untargeted metabolomics, biomechanical assays and live imaging. We will develop both CRISPR and small molecule-based approaches to attack the metabolic reprogramming in retinal disease