PO Box 9193
E230 WVU Eye Institute
1 Medical Center Drive
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- Rockefeller Neuroscience Institute
- Adjunct Faculty
- PhD, Wesleyan University, 1998
Kirschman LT*, Kolandaivelu S, Frederick JM, Dang L, Goldberg AF, Baehr W, Ramamurthy V. The Leber congenital amaurosis protein, AIPL1, is needed for the viability and functioning of cone photoreceptor cells. Hum Mol Genet. 2010 Mar 15;19(6):1076-87
Christiansen JR*, Kolandaivelu S, Bergo MO, Ramamurthy V. “RAS-converting enzyme 1-mediated endoproteolysis is required for trafficking of rod phosphodiesterase 6 to photoreceptor outer segments” Proc Natl Acad Sci U S A. 2011 May 24;108(21):8862-6.
Kolandaivelu S, Chang B, Ramamurthy V. Rod Phosphodiesterase-6 (PDE6) catalytic subunits restores cone function in a mouse model lacking cone PDE6 catalytic subunit. J Biol Chem. 2011 Jul 28.
Ku C*, Chiodo V, Boye S, Goldberg A, Li T, Hauswirth W, Ramamurthy V. Gene therapy using self-complementary Y733F capsid mutant AAV2/8 restores vision in a model of early onset Leber congenital amaurosis. Hum Mol Genet. 2011.
Christiansen, JR* and Ramamurthy V (2012) Greasing the protein trafficking machinery of photoreceptor neurons – Role for post prenylation processing of proteins, Invited Review. Cellular Logistics
The Ramamurthy lab aims to decipher the biochemical pathways that control the complex processing of information through neurons to the brain. We use the visual system as a model to comprehend this process. In vision, defects in light signal processing result in neuronal death and blindness. Several recent studies have established the link between mutations in various genes to blinding diseases. However, the functional role of these genes and why defects in these genes cause blindness remains elusive. In our research group, we use various molecular, biochemical and physiological approaches to probe the biochemical basis behind defects that cause the break down of the neuronal circuits and ultimately visual impairment. Our investigations are also critical in designing innovative therapeutic approaches in treating these neuronal degenerations.
Techniques Used in the Laboratory: Cloning, expression and purification of proteins in bacteria, insect and human cells; Creation of transgenic and knock out mouse models of disease; Analyses of protein complexes by immunoprecipitation, liquid chromatography and mass-spectrometry; protein localization by in-situs, confocal immunofluorscence and electron microscopy; Electrophysiology; Synthesis, folding and assembly of proteins studied by pulse-label, pulse-chase and immunoprecipitation; Protein structure-function relationship.
Keywords: Neuronal degeneration, Childhood Blindness, Congenital Stationary Night Blindness, Signal processing from retina to brain, Synaptic transmission, Ribbon Synpases, Visual cortex, Gene therapy, Small molecule therapy, Posttranslational modifcation of proteins and protein assembly