Timothy R Nurkiewicz, PhD

Professor and Chair

Organization:

West Virginia University School of Medicine

Department:

Physiology, Pharmacology & Toxicology

Classification:

Faculty

1. Stapleton PA, McBride CR, Yi J, Abukabda AB, Nurkiewicz TR. Estrous
cycle-dependent modulation of in vivo microvascular dysfunction after
nanomaterial inhalation. Reproductive toxicology (Elmsford, N.Y.). 2018;
78:20-28. NIHMSID: NIHMS973887 PubMed [journal] PMID: 29545171, PMCID: PMC6034709

2. Mandler WK, Nurkiewicz TR, Porter DW, Kelley EE, Olfert IM. Microvascular
dysfunction following multi-walled carbon nanotube exposure is mediated by
thrombospondin-1 receptor CD47. Toxicological sciences : an official journal of
the Society of Toxicology. 2018; PubMed [journal] PMID: 29788500

3. Nichols CE, Shepherd DL, Hathaway QA, Durr AJ, Thapa D, Abukabda A, Yi J,
Nurkiewicz TR, Hollander JM. Reactive oxygen species damage drives cardiac and
mitochondrial dysfunction following acute nano-titanium dioxide inhalation
exposure. Nanotoxicology. 2018; 12(1):32-48. NIHMSID: NIHMS929989 PubMed
[journal] PMID: 29243970, PMCID: PMC5777890

4. Stapleton PA, Hathaway QA, Nichols CE, Abukabda AB, Pinti MV, Shepherd DL,
McBride CR, Yi J, Castranova VC, Hollander JM, Nurkiewicz TR. Maternal engineered
nanomaterial inhalation during gestation alters the fetal transcriptome. Particle
and fibre toxicology. 2018; 15(1):3. PubMed [journal] PMID: 29321036, PMCID:
PMC5763571

5. Stefaniak AB, LeBouf RF, Duling MG, Yi J, Abukabda AB, McBride CR, Nurkiewicz TR.
Inhalation exposure to three-dimensional printer emissions stimulates acute
hypertension and microvascular dysfunction. Toxicology and applied pharmacology.
2017; 335:1-5. NIHMSID: NIHMS909372 PubMed [journal] PMID: 28942003, PMCID:
PMC5643255

6. Stefaniak AB, LeBouf RF, Yi J, Ham J, Nurkewicz T, Schwegler-Berry DE, Chen BT,
Wells JR, Duling MG, Lawrence RB, Martin SB Jr, Johnson AR, Virji MA.
Characterization of chemical contaminants generated by a desktop fused deposition
modeling 3-dimensional Printer. Journal of occupational and environmental
hygiene. 2017; 14(7):540-550. NIHMSID: NIHMS966182 PubMed [journal] PMID:
28440728, PMCID: PMC5967408

7. Hathaway QA, Nichols CE, Shepherd DL, Stapleton PA, McLaughlin SL, Stricker JC,
Rellick SL, Pinti MV, Abukabda AB, McBride CR, Yi J, Stine SM, Nurkiewicz TR,
Hollander JM. Maternal-engineered nanomaterial exposure disrupts progeny cardiac
function and bioenergetics. American journal of physiology. Heart and circulatory
physiology. 2017; 312(3):H446-H458. PubMed [journal] PMID: 28011589, PMCID:
PMC5402018

8. Mandler WK, Nurkiewicz TR, Porter DW, Olfert IM. Thrombospondin-1 mediates
multi-walled carbon nanotube induced impairment of arteriolar dilation.
Nanotoxicology. 2017; 11(1):112-122. NIHMSID: NIHMS844255 PubMed [journal] PMID:
28024456, PMCID: PMC5290593

9. Abukabda AB, Stapleton PA, Nurkiewicz TR. Metal Nanomaterial Toxicity Variations
Within the Vascular System. Current environmental health reports. 2016;
3(4):379-391. NIHMSID: NIHMS820186 PubMed [journal] PMID: 27686080, PMCID:
PMC5112123

10. Engler-Chiurazzi EB, Stapleton PA, Stalnaker JJ, Ren X, Hu H, Nurkiewicz TR,
McBride CR, Yi J, Engels K, Simpkins JW. Impacts of prenatal nanomaterial
exposure on male adult Sprague-Dawley rat behavior and cognition. Journal of
toxicology and environmental health. Part A. 2016; 79(11):447-52. NIHMSID:
NIHMS783351 PubMed [journal] PMID: 27092594, PMCID: PMC4899177

11. Ishikawa AA, Salazar JV, Salinas M, Gaitani CM, Nurkiewicz T, Negrete GR, Garcia
CD. Self-Assembled Nanospheres for Encapsulation and Aerosolization of
Rifampicin. RSC advances. 2016; 6(16):12959-12963. NIHMSID: NIHMS756787 PubMed
[journal] PMID: 26998252, PMCID: PMC4792303

12. Maurer MM, Donohoe GC, Maleki H, Yi J, McBride C, Nurkiewicz TR, Valentine SJ.
Comparative plasma proteomic studies of pulmonary TiO2 nanoparticle exposure in
rats using liquid chromatography tandem mass spectrometry. Journal of proteomics.
2016; 130:85-93. NIHMSID: NIHMS725097 PubMed [journal] PMID: 26375203, PMCID:
PMC4640999

13. Minarchick VC, Stapleton PA, Sabolsky EM, Nurkiewicz TR. Cerium Dioxide
Nanoparticle Exposure Improves Microvascular Dysfunction and Reduces Oxidative
Stress in Spontaneously Hypertensive Rats. Frontiers in physiology. 2015; 6:339.
PubMed [journal] PMID: 26635625, PMCID: PMC4646966

14. Stapleton PA, McBride CR, Yi J, Nurkiewicz TR. Uterine microvascular sensitivity
to nanomaterial inhalation: An in vivo assessment. Toxicology and applied
pharmacology. 2015; 288(3):420-8. NIHMSID: NIHMS725488 PubMed [journal] PMID:
26375943, PMCID: PMC4620930

15. Stapleton PA, Nichols CE, Yi J, McBride CR, Minarchick VC, Shepherd DL, Hollander
JM, Nurkiewicz TR. Microvascular and mitochondrial dysfunction in the female F1
generation after gestational TiO2 nanoparticle exposure. Nanotoxicology. 2015;
9(8):941-51. NIHMSID: NIHMS753753 PubMed [journal] PMID: 25475392, PMCID:
PMC4736545

16. Armstead AL, Minarchick VC, Porter DW, Nurkiewicz TR, Li B. Acute inflammatory
responses of nanoparticles in an intra-tracheal instillation rat model. PloS one.
2015; 10(3):e0118778. PubMed [journal] PMID: 25738830, PMCID: PMC4349695

17. Minarchick VC, Stapleton PA, Fix NR, Leonard SS, Sabolsky EM, Nurkiewicz TR.
Intravenous and gastric cerium dioxide nanoparticle exposure disrupts
microvascular smooth muscle signaling. Toxicological sciences : an official
journal of the Society of Toxicology. 2015; 144(1):77-89. PubMed [journal] PMID:
25481005, PMCID: PMC4349137

18. Donohoe GC, Maleki H, Arndt JR, Khakinejad M, Yi J, McBride C, Nurkiewicz TR,
Valentine SJ. A new ion mobility-linear ion trap instrument for complex mixture
analysis. Analytical chemistry. 2014; 86(16):8121-8. NIHMSID: NIHMS779660 PubMed
[journal] PMID: 25068446, PMCID: PMC4845737

19. Stapleton PA, Nurkiewicz TR. Vascular distribution of nanomaterials. Wiley
interdisciplinary reviews. Nanomedicine and nanobiotechnology. 2014; 6(4):338-48.
NIHMSID: NIHMS615765 PubMed [journal] PMID: 24777845, PMCID: PMC4135432

20. Stapleton PA, Nurkiewicz TR. Maternal nanomaterial exposure: a double threat to
maternal uterine health and fetal development? Nanomedicine (London, England).
2014; 9(7):929-31. NIHMSID: NIHMS617985 PubMed [journal] PMID: 24978457, PMCID:
PMC4128482

21. Nanoparticle Aerosol Generator
Yi J, Nurkiewicz TR.
United States Patent #8,881,997.
My Bibliography [patent]

22. Minarchick VC, Stapleton PA, Porter DW, Wolfarth MG, Çiftyürek E, Barger M,
Sabolsky EM, Nurkiewicz TR. Pulmonary cerium dioxide nanoparticle exposure
differentially impairs coronary and mesenteric arteriolar reactivity.
Cardiovascular toxicology. 2013; 13(4):323-37. NIHMSID: NIHMS476065 PubMed
[journal] PMID: 23645470, PMCID: PMC3796163

23. Stapleton PA, Minarchick VC, Yi J, Engels K, McBride CR, Nurkiewicz TR. Maternal
engineered nanomaterial exposure and fetal microvascular function: does the
Barker hypothesis apply? American journal of obstetrics and gynecology. 2013;
209(3):227.e1-11. NIHMSID: NIHMS474599 PubMed [journal] PMID: 23643573, PMCID:
PMC3757100

24. Yi J, Chen BT, Schwegler-Berry D, Frazer D, Castranova V, McBride C, Knuckles TL,
Stapleton PA, Minarchick VC, Nurkiewicz TR. Whole-body nanoparticle aerosol
inhalation exposures. Journal of visualized experiments : JoVE. 2013;
(75):e50263. PubMed [journal] PMID: 23685643, PMCID: PMC3679583

25. Hubbs AF, Sargent LM, Porter DW, Sager TM, Chen BT, Frazer DG, Castranova V,
Sriram K, Nurkiewicz TR, Reynolds SH, Battelli LA, Schwegler-Berry D, McKinney W,
Fluharty KL, Mercer RR. Nanotechnology: toxicologic pathology. Toxicologic
pathology. 2013; 41(2):395-409. NIHMSID: NIHMS723787 PubMed [journal] PMID:
23389777, PMCID: PMC4665093

26. Knuckles TL, Stapleton PA, Minarchick VC, Esch L, McCawley M, Hendryx M,
Nurkiewicz TR. Air pollution particulate matter collected from an Appalachian
mountaintop mining site induces microvascular dysfunction. Microcirculation (New
York, N.Y. : 1994). 2013; 20(2):158-69. NIHMSID: NIHMS739166 PubMed [journal]
PMID: 22963349, PMCID: PMC4852712

27. Knuckles TL, Yi J, Frazer DG, Leonard HD, Chen BT, Castranova V, Nurkiewicz TR.
Nanoparticle inhalation alters systemic arteriolar vasoreactivity through
sympathetic and cyclooxygenase-mediated pathways. Nanotoxicology. 2012;
6(7):724-35. NIHMSID: NIHMS712046 PubMed [journal] PMID: 21830860, PMCID:
PMC4850922

28. Stapleton PA, Minarchick VC, Cumpston AM, McKinney W, Chen BT, Sager TM, Frazer
DG, Mercer RR, Scabilloni J, Andrew ME, Castranova V, Nurkiewicz TR. Impairment
of coronary arteriolar endothelium-dependent dilation after multi-walled carbon
nanotube inhalation: a time-course study. International journal of molecular
sciences. 2012; 13(11):13781-803. PubMed [journal] PMID: 23203034, PMCID:
PMC3509550

29. Stapleton PA, Minarchick VC, McCawley M, Knuckles TL, Nurkiewicz TR. Xenobiotic
particle exposure and microvascular endpoints: a call to arms. Microcirculation
(New York, N.Y. : 1994). 2012; 19(2):126-42. NIHMSID: NIHMS327179 PubMed
[journal] PMID: 21951337, PMCID: PMC3257352

30. Kang LS, Nurkiewicz TR, Wu G, Boegehold MA. Changes in eNOS phosphorylation
contribute to increased arteriolar NO release during juvenile growth. American
journal of physiology. Heart and circulatory physiology. 2012; 302(3):H560-6.
PubMed [journal] PMID: 22140037, PMCID: PMC3353788

31. Nurkiewicz TR, Porter DW, Hubbs AF, Stone S, Moseley AM, Cumpston JL, Goodwill
AG, Frisbee SJ, Perrotta PL, Brock RW, Frisbee JC, Boegehold MA, Frazer DG, Chen
BT, Castranova V. Pulmonary particulate matter and systemic microvascular
dysfunction. Research report (Health Effects Institute). 2011; (164):3-48. PubMed
[journal] PMID: 22329339

32. Hubbs AF, Mercer RR, Benkovic SA, Harkema J, Sriram K, Schwegler-Berry D,
Goravanahally MP, Nurkiewicz TR, Castranova V, Sargent LM. Nanotoxicology--a
pathologist's perspective. Toxicologic pathology. 2011; 39(2):301-24. PubMed
[journal] PMID: 21422259

33. LeBlanc AJ, Moseley AM, Chen BT, Frazer D, Castranova V, Nurkiewicz TR.
Nanoparticle inhalation impairs coronary microvascular reactivity via a local
reactive oxygen species-dependent mechanism. Cardiovascular toxicology. 2010;
10(1):27-36. NIHMSID: NIHMS172259 PubMed [journal] PMID: 20033351, PMCID:
PMC2825710

34. Nurkiewicz TR, Wu G, Li P, Boegehold MA. Decreased arteriolar tetrahydrobiopterin
is linked to superoxide generation from nitric oxide synthase in mice fed high
salt. Microcirculation (New York, N.Y. : 1994). 2010; 17(2):147-57. NIHMSID:
NIHMS226869 PubMed [journal] PMID: 20163541, PMCID: PMC3402363

35. Nurkiewicz TR, Porter DW, Hubbs AF, Stone S, Chen BT, Frazer DG, Boegehold MA,
Castranova V. Pulmonary nanoparticle exposure disrupts systemic microvascular
nitric oxide signaling. Toxicological sciences : an official journal of the
Society of Toxicology. 2009; 110(1):191-203. PubMed [journal] PMID: 19270016,
PMCID: PMC2696324

36. LeBlanc AJ, Cumpston JL, Chen BT, Frazer D, Castranova V, Nurkiewicz TR.
Nanoparticle inhalation impairs endothelium-dependent vasodilation in
subepicardial arterioles. Journal of toxicology and environmental health. Part A.
2009; 72(24):1576-84. NIHMSID: NIHMS142196 PubMed [journal] PMID: 20077232,
PMCID: PMC2808198

37. Nurkiewicz TR, Porter DW, Hubbs AF, Cumpston JL, Chen BT, Frazer DG, Castranova
V. Nanoparticle inhalation augments particle-dependent systemic microvascular
dysfunction. Particle and fibre toxicology. 2008; 5:1. PubMed [journal] PMID:
18269765, PMCID: PMC2276228

38. Prisby RD, Muller-Delp J, Delp MD, Nurkiewicz TR. Age, gender, and hormonal
status modulate the vascular toxicity of the diesel exhaust extract
phenanthraquinone. Journal of toxicology and environmental health. Part A. 2008;
71(7):464-70. PubMed [journal] PMID: 18306094

39. Hendryx M, Ahern MM, Nurkiewicz TR. Hospitalization patterns associated with
Appalachian coal mining. Journal of toxicology and environmental health. Part A.
2007; 70(24):2064-70. PubMed [journal] PMID: 18049995

40. Nurkiewicz TR, Boegehold MA. High salt intake reduces endothelium-dependent
dilation of mouse arterioles via superoxide anion generated from nitric oxide
synthase. American journal of physiology. Regulatory, integrative and comparative
physiology. 2007; 292(4):R1550-6. PubMed [journal] PMID: 17138723

41. Nurkiewicz TR, Porter DW, Barger M, Millecchia L, Rao KM, Marvar PJ, Hubbs AF,
Castranova V, Boegehold MA. Systemic microvascular dysfunction and inflammation
after pulmonary particulate matter exposure. Environmental health perspectives.
2006; 114(3):412-9. PubMed [journal] PMID: 16507465, PMCID: PMC1392236

42. Marvar PJ, Nurkiewicz TR, Boegehold MA. Reduced arteriolar responses to skeletal
muscle contraction after ingestion of a high salt diet. Journal of vascular
research. 2005; 42(3):226-36. PubMed [journal] PMID: 15855795

43. Nurkiewicz TR, Boegehold MA. Calcium-independent release of endothelial nitric
oxide in the arteriolar network: onset during rapid juvenile growth.
Microcirculation (New York, N.Y. : 1994). 2004; 11(6):453-62. PubMed [journal]
PMID: 15371127

44. Nurkiewicz TR, Porter DW, Barger M, Castranova V, Boegehold MA. Particulate
matter exposure impairs systemic microvascular endothelium-dependent dilation.
Environmental health perspectives. 2004; 112(13):1299-306. PubMed [journal] PMID:
15345343, PMCID: PMC1247520

45. Davis MJ, Wu X, Nurkiewicz TR, Kawasaki J, Gui P, Hill MA, Wilson E. Regulation
of ion channels by integrins. Cell biochemistry and biophysics. 2002;
36(1):41-66. PubMed [journal] PMID: 11939371

46. Davis MJ, Wu X, Nurkiewicz TR, Kawasaki J, Gui P, Hill MA, Wilson E. Regulation
of ion channels by protein tyrosine phosphorylation. American journal of
physiology. Heart and circulatory physiology. 2001; 281(5):H1835-62. PubMed
[journal] PMID: 11668044

47. Davis MJ, Wu X, Nurkiewicz TR, Kawasaki J, Davis GE, Hill MA, Meininger GA.
Integrins and mechanotransduction of the vascular myogenic response. American
journal of physiology. Heart and circulatory physiology. 2001; 280(4):H1427-33.
PubMed [journal] PMID: 11247750

48. Nurkiewicz TR, Boegehold MA. Reinforcement of arteriolar myogenic activity by
endogenous ANG II: susceptibility to dietary salt. American journal of
physiology. Heart and circulatory physiology. 2000; 279(1):H269-78. PubMed
[journal] PMID: 10899066

49. Nurkiewicz TR, Boegehold MA. Limitation of arteriolar myogenic activity by local
nitric oxide: segment-specific effect of dietary salt. The American journal of
physiology. 1999; 277(5 Pt 2):H1946-55. PubMed [journal] PMID: 10564151

50. Nurkiewicz TR, Boegehold MA. High dietary salt alters arteriolar myogenic
responsiveness in normotensive and hypertensive rats. The American journal of
physiology. 1998; 275(6 Pt 2):H2095-104. PubMed [journal] PMID: 9843809

Mechanisms contributing to the systemic microvascular dysfunction that is triggered by pulmonary exposure to nanoparticles and ambient particulate matter (microvascular/environmental toxicology). These include: local regulation of blood flow, endotheli...

About Dr. Nurkiewicz.

Human exposures to xenobiotic particles continue to increase in both their frequency and diversity. Inhalation of particulate matter air pollution, and/or anthropogenically derived nanomaterials results in cardiovascular morbidity and mortality. Despite its importance in most every physiologic system, development and pathology; our understanding of how the microcirculation is affected by xenobiotic particle exposures is poor.

The greater goal of my research program is to characterize the systemic microvascular effects of inhaled particles (cardiovascular toxicology). This broad interest is pursued from two perspectives. First, the influence of engineered nanomaterial exposures on microvascular function is a primary focus of my research (nanotoxicology). Secondly (but equally), the influence of environmental air pollution particulate matter (environmental toxicology) is evaluated via exposures to a variety of point (mountain top mining) and mobile (combustion engine) source particles. Inhalation exposures are the main exposure route (aerosol generation). As a toxicologist, real-time aerosol characterization, exposure time-courses and dose-response relationships are critical components of our studies.

My research program is robustly committed to investigating how gender influences microvascular outcomes of particle exposure; or how sensitive populations, such as those with pre-existing conditions (e.g. pregnancy) or disease (e.g. hypertension) differentially respond to xenobiotics. This is most evident in our maternal and fetoplacental outcomes-based research, also known as the Developmental Origins of Health and Disease. In all our endeavors, we actively pursue the mechanisms that link pulmonary exposures with systemic biologic effects. Most notably, we investigate inflammatory and neurogenic pathways. To these ends, we are committed to preventing untoward xenobiotic exposures, or decreasing the severity of their impacts; as well as identifying the safe characteristics of nanomaterials for their use in diverse biomedical applications to ultimately benefit human health.